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A simple direct-addition microtitre plate enzymeimmunoassay (EIA) for progesterone in whole milk is described. The assay used antiserum raised against 11 alpha-hydroxyprogesterone 11-hemisuccinate (progesterone 11-hemisuccinate) and a heterologous label prepared by conjugation of 11 alpha-hydroxyprogesterone 11-glucuronide (progesterone 11-glucuronide) with(More)
Water availability is one of the most important limiting factors in agriculture worldwide, particularly in arid and semiarid regions. Six spring wheat genotypes, i.e. three UK cultivars Cadenza, Paragon, and Xi-19 and three synthetic-derived lines L-22, L-24, and L-38, were grown in a phytotron under well-watered (until 40 days after sowing) and drought(More)
A sensitive enzymeimmunoassay has been developed for measuring progesterone in unextracted bovine milk. An N-hydroxysuccinimide ester of 11 alpha -hydroxyprogesterone 11-hemisuccinate has been synthesised and used to form conjugates with beta-galactosidase in buffer at pH 7.0. The degree of incorporation of progesterone into the enzyme was demonstrated(More)
OBJECTIVES This study evaluated the effectiveness of cardiac counseling and rehabilitation programs led by a nurse counselor, compared with normal care on outcomes for myocardial infarction (MI) patients and their partners. METHODS A randomized controlled trial with follow-up to 1 year was conducted with 100 patients recruited within 72 hours of a first(More)
Grain yield (GY) and grain protein concentration (GPC) are two major traits contributing to the economic value of the wheat crop. These are, consequently, major targets in wheat breeding programs, but their simultaneous improvement is hampered by the negative correlation between GPC and GY. Identifying the genetic determinants of GPC and GY through(More)
OBJECTIVES To assess myocardial infarction (MI) patients' causal beliefs, how they change over time and the effects of different assessment methods. METHOD The study used a longitudinal design with a follow-up to 1 year. The participants were 100 MI patients who were recruited within 72 hours of admission. Each participant was interviewed and their causal(More)
A radioimmunoassay was developed for rapid determination of estradiol-17 beta concentrations in unextracted defatted bovine milk. The assay was dependent on the use of a highly specific anti-estradiol-17 beta antiserum. Application of a formula to correct for the interference associated with individual milk samples and use of appropriate assay blanks(More)
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