John Fitzmaurice

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Alternative policies to address global climate change are being debated in many nations and within the United Nations Framework Convention on Climate Change. To help provide objective and comprehensive analyses in support of this process, we have developed a model of the global climate system consisting of coupled sub-models of economic growth and(More)
Real-time PCR assays, based on hybridisation probes and LightCycler technology, were developed for VT 1 and VT 2 genes and applied to the detection and quantitation of DNA and mRNA of Escherichia coli O157:H7. The qualitative consensus PCR assay for the detection of VT 1 and/or VT 2 genes had a detection limit of 100 fg of E. coli O157:H7 genomic DNA and(More)
In this study, enrichment procedures and two recovery methods, a membrane surface adhesion technique and an immunomagnetic separation (IMS), were compared for use in conjunction with a multiplex polymerase chain reaction (PCR) method with a view to describing a fast (24 h) and economical test for detection of Escherichia coli O157:H7 in meat samples. The(More)
A new technique is developed for modeling 3D permeability distributions. The technique integrates all available data into a fluid flow simulation model. The integrated modeling process honors the essential aspects of the established reservoir descriptions as well as the geological facies model and engineering data. The added value of data integration of the(More)
The concept of three-colour detection through a single pixel contact is demonstrated. An intermediate absorber layer is inserted into the centre of the typical back-to-back junction design for two-colour detection. Bias dependent electronic barriers around this intermediate layer control its contribution to the overall signal. One critical parameter for(More)
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