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In this study, we have used a mouse monoclonal antibody to rat Ia (RT1-B or class II) antigens to demonstrate, by immunofluorescence on frozen sections, intensely Ia-positive dendritic cells in the interstitial connective tissues of every tissue we have examined (heart, liver, thyroid, pancreas, skin, kidney, ureter, and bladder) with the striking exception(More)
The major histocompatibility complex (MHC) of the rat is as yet poorly defined. Serological studies with recombinant strains have shown that class I genes are present on both sides of the class II region, as is the case for the mouse MHC (Butcher and Howard 1986). Most cytotoxic T-cell responses map to the RT1.A region (Gtinther and Wurst 1984), thus(More)
Phorbol esters rapidly induce aggregation of human mononuclear leukocytes in vitro. Previous studies have indicated that cell surface proteins are involved. We report now that the monoclonal antibody 60.3, either as purified IgG or as Fab' fragments, to an antigen common to leukocytes completely inhibited the phorbol ester-induced intercellular adhesion(More)
Identity management refers to the process of representing and recognising entities as digital identities in computer networks. Authentication, which is an integral part of identity management, serves to verify claims about holding specific identities. Identity management is therefore fundamental to, and sometimes include, other security constructs such as(More)
We have used a monoclonal antibody, PA2.6, directed against the heavy chain of HLA-ABC antigens to study the detailed tissue distribution of MHC class I antigens. Normal tissues from throughout the human body were obtained fresh from organ donors or operative specimens and were snap-frozen in liquid nitrogen within 1-2 hr of removal. Frozen sections were(More)
Spleen cells from a BALB/c mouse that had been immunized with human thymocytes were fused with the myeloma line P3-NS 1/1 Ag 4.1. One of the resulting hybrid clones (NA 1/34) secreted an antibody that was highly specific for human thymocytes. Eighty-five % of thymocytes expressed the antigen designated HTA1. There were an estimated 15 x 10(4) molecules of(More)
The alloantibody response of LEW rats immunized with DA kidney homogenate or given vascularized DA kidney grafts was studied using an 125I-anti-Ig binding assay with kidney homogenate as the target tissue. A major component of the sera was found to be directed at a kidney-specific alloantigen. Analysis of LEW X (DA X LEW) F1 backcross rats showed that the(More)
The monoclonal antibody (F 10-44-2) described in this report recognizes an antigen which by quantitative absorption analysis is found predominantly on spleen, lymph node, bone marrow, thymus, granulocytes and brain, the amount of antigen on these tissues being approximately the same within a factor of 2 or 3. Analysis with the fluorescence-activated cell(More)
Comparisons of rat class I DNA sequences with each other and with mouse class I genes have revealed a higher level of similarity between different rat class I genes than between rat and mouse class I genes (Rada et al. 1990; Mauxion et al. 1989). These results have led to the proposal that rat class I genes form a discrete family of genes, between which(More)
DA (RT1a) hearts were transplanted into PVG (RT1c) or DA recipients, excised on days 1, 3, 5, or 7 after grafting, and examined by immunohistological techniques and quantitative absorption analyses, using allospecific mouse anti-rat class I and class II major histocompatibility complex (MHC) monoclonal antibodies. Cryostat sections stained by the peroxidase(More)