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Brunescent lenses and normal human lenses more than 70 years old exhibit red fluorescence due to a fluorophor with emission maximum at 672 nm under excitation by the 647.1 nm line of krypton ion laser. The properties and mode of occurrence of this fluorophor suggest that its formation is highly pertinent to senile nuclear pathology.
Lipid peroxidation has been shown to be involved in the pathogenesis of some types of cataract. The possibility of such a mechanism was investigated in Emory mouse cataract. Malondialdehyde, a breakdown product of lipid peroxides, increased 4-fold in advanced cataract. Studies on cation transport revealed that in early cataract there was no alteration in(More)
The mechanism of oxidative damage to the lens through intraocular photochemical generation of superoxide and its derivatization to other oxidants such as singlet oxygen, hydroxyl radical and hydrogen peroxide has been studied. Rat lenses when organ cultured aerobically in TC 199 containing additional amounts of riboflavin were damaged as demonstrated by an(More)
The late onset cataract of the Emory mouse has appealed to many investigators as a useful animal model for human senile cataract. It has been the subject of about 15 publications, beginning in 1982. These have explored many features, including histology, chromatography and isoelectric focusing of the crystallins, enzyme profiles, amino acid and ion(More)
The authors have put quantitation of human lens fluorescence on a rational basis by using the accompanying Raman signal from lens protein as a normalization factor. The intensity ratio, Fluorescence/Raman (F/R), may be used to compare lenses of different ages when the exciting wavelength is long enough to give a measurable Raman signal. In younger lenses(More)
The Emory mouse develops a late-appearing hereditary cataract having many characteristics which suggest its usefulness as an animal model for human senile cataract. This paper presents some results of analyses designed to determine biochemical changes associated with initiation and development of the cataract. The measurements carried out include(More)
Antisera against synthetic peptides corresponding to various regions of the Main Intrinsic Polypeptide (MIP26K) of fiber lens membranes have been used to probe Western blots of Emory mouse lens proteins resolved by SDS-polyacrylamide gel electrophresis. When compared with clear lenses from control animals of approximately the same age, the MIP26K component(More)
Exopeptidases identified as dipeptidyl peptidase III and leucine aminopeptidase, and an endopeptidase, prolyl endopeptidase, were found in the Emory Mouse cataract and the Cataract Resistant mouse lens extracts. The specific activity measured on Arg-Arg-2-NNap for DPP III and the hydrolysis of Boc-Arg-Pro-2-NNap for prolyl endopeptidase were higher in the(More)
We determined the conditions required for the establishment of lens epithelial cell lines from individual Emory and age matched cataract-resistant (CR) mice, and investigated the response of these cells to hydrogen peroxide. The technique described here permits the establishment of mouse lens epithelial cell lines from individual animals and provides an(More)