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Botrytis cinerea, an important fungal plant pathogen, secretes aspartic proteinase (AP) activity in axenic cultures. No cysteine, serine or metalloproteinase activity could be detected. Proteinase activity was higher in culture medium containing BSA or wheat germ extract, as compared to minimal medium. A proportion of the enzyme activity remained in the(More)
BACKGROUND Phytophthora species are oomycete plant pathogens with such major social and economic impact that genome sequences have been determined for Phytophthora infestans, P. sojae and P. ramorum. Pepsin-like aspartic proteinases (APs) are produced in a wide variety of species (from bacteria to humans) and contain conserved motifs and landmark residues.(More)
The structures of the native Saccharomyces cerevisiae proteinase A have been solved by molecular replacement in the monoclinic and trigonal crystal forms and refined at 2.6-2.7A resolution. These structures agree overall with those of other uninhibited aspartic proteinases. However, an unusual orientation for the side chain of Tyr75, a conserved residue on(More)
The Nebraska Historic Buildings Survey (NEHBS) is an ongoing project of the State Historic Preservation Office. Since its beginnings in 1974 with limited fieldwork by staff and student interns, NEHBS has expanded from a few thousand sites in urban and rural areas to over 40,000 recorded properties in three-fourths of the state. By 1992, the office plans to(More)
1 During the last four decades governments in wealthy countries have built up large contingent liabilities due to the implicit guarantees they have provided to their financial sectors. Politicians are motivated to create near term growth and always reluctant to permit hardships that would otherwise arise from defaults and greater austerity. As a result, the(More)
The A1 family of eukaryotic aspartic proteinases (APs) forms one of the 16 AP families. Although one of the best characterized families, the recent increase in genome sequence data has revealed many fungal AP homologs with novel sequence characteristics. This study was performed to explore the fungal AP sequence space and to obtain an in-depth understanding(More)
PRELIMINARY VERSION—Not to be quoted or cited. This paper was produced by the Growth Commission's Secretariat to inform the thinking of the Commissioners. The analysis does not necessarily reflect the views of the Commissioners. In contrast, the proposals are those of the Growth Commission report.