John D. Delaney

Learn More
The increasing prevalence of automated image acquisition systems is enabling new types of microscopy experiments that generate large image datasets. However, there is a perceived lack of robust image analysis systems required to process these diverse datasets. Most automated image analysis systems are tailored for specific types of microscopy, contrast(More)
The post genomic era introduced the need to define single gene functions within biological pathways. A systems biology approach can be realized by automating image acquisition and phenotype classification. While machinery for automated data acquisition have been developing rapidly in the past years, the main bottleneck remains the effectiveness of the(More)
Paget's disease of bone (PDB) is a focal disorder of bone remodeling that leads to overgrowth of affected bone, with rare progression to osteosarcoma. Extensive studies of familial PDB showed that a majority of cases harbor germline mutations in the Sequestosome1 gene (SQSTM1). In contrast, little is known about the mutational status of SQSTM1 in sporadic(More)
We present an initial molecular characterization of a morphological transition between two early aging states. In previous work, an age score reflecting physiological age was developed using a machine classifier trained on images of worm populations at fixed chronological ages throughout their lifespan. The distribution of age scores identified three stable(More)
We present results from machine classification of melanoma biopsies sectioned and stained with hematoxylin/eosin (H&E) on tissue microarrays (TMA). The four stages of melanoma progression were represented by seven tissue types, including benign nevus, primary tumors with radial and vertical growth patterns (stage I) and four secondary metastatic tumors:(More)
Extracellular inorganic phosphate (P(i)) concentrations are the highest in the growth plate just before the onset of mineralization. The study reported here demonstrates that P(i) not only is required for hydroxyapatite mineral formation but also modulates terminal differentiation and apoptosis of growth plate chondrocytes. Extracellular P(i) stimulated(More)
Pattern recognition techniques can potentially be used to quantitatively analyze a wide variety of biomedical images. A challenge in applying this methodology is that biomedical imaging uses many imaging modalities and subjects. Pattern recognition relies on numerical image descriptors (features) to describe image content. Thus, the application of pattern(More)
  • 1