John Brendan M Mullen

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OBJECTIVES To analyze the relationship between DNA damage and standard semen parameters (SSP) in patients who present for fertility evaluation. Evaluation of male fertility includes assessment of the SSP and increasingly sperm DNA damage. However, the relationship between DNA damage and SSP remains controversial. METHODS Following Institutional Research(More)
The objective of this study was to examine the relationship between DNA integrity and protamines in human sperm. One hundred forty-nine male infertility patients were included in an Institutional Review Board-approved study. Sperm were evaluated for DNA fragmentation using the DNA Integrity Assay, a test equivalent to the sperm chromatin structure assay(More)
OBJECTIVE To determine the incidence of bacteriospermia and elevated seminal leukocytes (ESL) in a subfertile male population and correlate these results with semen parameters. DESIGN Retrospective cohort study. SETTING Canadian tertiary-level male infertility clinic and university-affiliated andrology and microbiology laboratories. PATIENT(S) Four(More)
OBJECTIVES This study was designed to document the association of endocardial fibroelastosis (EFE) and maternal autoantibodies. BACKGROUND Neonatal lupus erythematosus is associated with the transplacental passage of maternal anti-Ro and anti-La antibodies, leading to complete atrioventricular block (CAVB). In some cases, CAVB is associated with EFE.(More)
BACKGROUND Maternal anti-Ro and anti-La antibodies are associated with congenital heart block (CHB). Although endocardial fibroelastosis (EFE) has been described in isolated cases of autoantibody-mediated CHB, the natural history and pathogenesis of this disease are poorly understood. METHODS AND RESULTS We retrospectively reviewed the clinical history,(More)
The purpose of our study was to analyze the relationship of leukocytospermia to sperm DNA damage as assessed by the DNA integrity assay. Results indicate that leukocytospermia has a significant negative effect on the standard semen parameters of concentration, motility, and morphology but at most a very weak effect on DNA integrity as measured by the DNA(More)
OBJECTIVE To determine the utility and cost of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) screening in infertile males. DESIGN Cohort study. SETTING Canadian tertiary-level male infertility clinic and university-affiliated laboratories. PATIENT(S) 5,588 male infertility patients. INTERVENTION(S) CT and NG testing on 8,972 urine and(More)
Telomeres play a fundamental role in the organization of the sperm nucleus resulting in the looped chromosome configuration and non-random positioning of chromosomes. Telomeres localize in the nuclear periphery and interact dynamically by forming dimers and tetramers. The purpose of this study was to evaluate the relationship of telomere interactions to DNA(More)
OBJECTIVE To compare DNA damage in ejaculated and testicular spermatozoa in patients with previously unsuccessful oral antioxidant treatment. DESIGN Prospective clinical study. SETTING University-affiliated teaching hospital. PATIENT(S) Twelve men with persistently high sperm DNA damage. INTERVENTION(S) Evaluation of DNA damage of ejaculated and(More)