John B. Pierce

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The diurnal change in activity of ribulose 1,5-bisphosphate (Rbu-1,5-P(2)) carboxylase [3-phospho-D-glycerate carboxy-lyase (dimerizing); EC 4.1.1.39] of leaves of Phaseolus vulgaris is regulated (in part) by mechanisms that control the level of an endogenous inhibitor that binds tightly to the activated (carbamoylated) form of Rbu-1,5-P(2) carboxylase.(More)
Human adipose tissue is an ideal autologous source of mesenchymal stem cells (MSCs) for various regenerative medicine and tissue engineering strategies. Aged patients are one of the primary target populations for many promising applications. It has long been known that advanced age is negatively correlated with an organism’s reparative and regenerative(More)
NAKAYAMA,TERUO, H.T. HAMMEL, J.D. HARDY, AND J.S. EISENMAN. Thermal stimulation of electrd activity of single units of the preoptic region. Am. J. Physiol. 204(6) : I 122-1126. 1g63.-Single unit activity has been recorded in the hypothalamus and preoptic region of cats anesthetized with urethan during local hypothalamic heating and cooling. Heating was(More)
A s CO2 concentrations continue to rise and discussions on global warming assume ever more warning tones, understanding of the CO2 cycle between the atmosphere and biosphere becomes ever more important. Most CO2 enters the biosphere through the actions of Rubisco, an enzyme found in all plants and many microorganisms. For many of these organisms, this entry(More)
Carbonyl sulfide, a competitive inhibitor of ribulose-bisphosphate carboxylase with respect to CO2 (Laing, W. A., and Christeller, J. T. (1980) Arch. Biochem. Biophys. 202, 592-600), is an alternate substrate. Thiocarboxylation was monitored by mass spectrometry as the stoichiometric consumption of carbonyl sulfide. The product, 1-thio-3-phosphoglycerate,(More)
The carboxylated, 6-carbon reaction intermediate (3-keto-2-carboxyarabinitol 1,5-bisphosphate) from the ribulose-1,5-bisphosphate carboxylase reaction was obtained by denaturing the enzyme with acid during steady-state turnover. Carbon-13 NMR analysis indicates that this beta-keto acid exists in solution predominantly as the C-3 ketone (as opposed to the(More)
Recent advances in the fields of neuroscience, computer science, and biomedical engineering now allow for the analysis of large-scale neurophysiological data sets to be carried out on-line and in real time. Here, we described an on-going effort in our research laboratory to build a computer system that will allow for on-line, real-time analyses of the(More)
Signal transduction through the interferongamma (IFNgamma) receptor involves the formation of a ligand-dependent multimolecular association of receptor chains (alpha and beta), Janus tyrosine kinases (Jak1 and Jak2), and the transcription factor (signal transducers and activators of transcription 1alpha (STAT1alpha)) in addition to activation of(More)
Activation of early response genes by interferons (IFNs) and other cytokines requires tyrosine phosphorylation of a family of transcription factors termed signal transducers and activators of transcription (Stats). The Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) is required for cytokine-induced tyrosine phosphorylation and dimerization of(More)
BACKGROUND Human adipose tissue (AT) is an ideal stem cell source for autologous cell-based therapies. The preferred setting for tissue engineering and regenerative medicine applications is the availability of clinically acceptable off-the-shelf cells and cell products. As AT is not always available for use, cryopreserved tissue represents an alternative(More)