John A Jellies

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Octopamine, a biogenic amine analogous to norepinephrine, plays an important role in the orchestration and modulation of invertebrate behavior. In the leech, the behavioral actions of octopamine have been demonstrated; however, identification of octopaminergic neurons had not been determined by using immunohistochemical techniques. Thus, we used an antibody(More)
Using monoclonal antibodies, we have identified two novel intermediate filament (IF) proteins, Gliarin and Macrolin, which are specifically expressed in the central nervous system of an invertebrate. The two proteins both contain the coiled-coil rod domain typical of the superfamily of IF proteins flanked by unique N- and C-terminal domains. Gliarin was(More)
In leech, the central projections of peripheral sensory neurons segregate into specific axonal tracts, which are distinguished by differential expression of surface antigens recognized by the monoclonal antibodies Lan3-2 and Lan4-2. Lan3-2 recognizes an epitope expressed on axons that segregate into three distinct axon fascicles. In contrast, the(More)
Segmental variation in identified neurons may provide an opportunity to examine extrinsic influences on neuronal phenotype, since segmentally homologous neurons must contain much the same intrinsic information, having arisen from very similar or identical precursors. Two large serotonergic Retzius (Rz) cells are found in each segmental ganglion of the leech(More)
By immunoaffinity purification with the mAb Lan3-2, we have identified two novel Ig superfamily members, Tractin and LeechCAM. LeechCAM is an NCAM/FasII/ApCAM homologue, whereas Tractin is a cleaved protein with several unique features that include a PG/YG repeat domain that may be part of or interact with the extracellular matrix. Tractin and LeechCAM are(More)
Invertebrates have proved to be important experimental systems for examining questions related to growth cone navigation and nerve formation, in large part because of their simpler nervous systems. However, such apparent simplicity can be deceiving because the final stereotyped patterns may be the result of multiple developmental mechanisms and not(More)
By labeling the germinal plates of staged leech embryos with monoclonal antibodies to the immunoglobulin superfamily member Tractin, we have documented the distribution and initial development of peripheral neurons in a hirudinid leech. We find, in addition to sensillar and extrasensillar sensory neurons, that there are 21 identifiable peripheral neurons in(More)
The present study examines the morphological development of a highly organized muscle layer in the leech Hirudo medicinalis, in an effort to characterize those factors that are important in directing its assembly. The tubular body wall of the leech contains 3 major muscle layers that are anatomically distinct: an inner layer of longitudinal muscle, an outer(More)
Muscle development has been the subject of intense scrutiny at cellular, biochemical and molecular levels, yet little is known about the factors that generate anatomically distinct muscles during embryogenesis. We now know that at least some muscles are initially organized by interactions with particular cells that appear early in development, the muscle(More)
In most segments of the leech, a pair of Retzius (Rz) cells innervate the body wall musculature and skin; however, in the segments specialized for reproduction (midbody segments 5 and 6), these neurons innervate the reproductive tissue instead. Whereas all Rz cells have the same morphology early in embryogenesis, those in the reproductive segments [Rz(5,6)](More)