John A. Hangasky

We don’t have enough information about this author to calculate their statistics. If you think this is an error let us know.
Learn More
Nonheme Fe(II)/αKG-dependent oxygenases catalyze diverse reactions, typically inserting an O atom from O2 into a C-H bond. Although the key to their catalytic cycle is the fact that binding and positioning of primary substrate precede O2 activation, the means by which substrate binding stimulates turnover is not well understood. Factor Inhibiting HIF (FIH)(More)
Factor inhibiting HIF (FIH) is a cellular O 2-sensing enzyme, which hydroxylates the hypoxia inducible factor-1α. Previously reported inverse solvent kinetic isotope effects indicated that FIH limits its overall turnover through an O 2 activation step (2013) Biochemistry 52, 1594−1602). Here we characterize the rate-limiting step for O 2 activation by FIH(More)
  • 1