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All eukaryotic cells present at the cell surface a specific set of plasma membrane proteins that modulate responses to internal and external cues and whose activity is also regulated by protein degradation. We characterized the lytic vacuole-dependent degradation of membrane proteins in Arabidopsis thaliana by means of in vivo visualization of vacuolar(More)
Cell polarity reflected by asymmetric distribution of proteins at the plasma membrane is a fundamental feature of unicellular and multicellular organisms. It remains conceptually unclear how cell polarity is kept in cell wall-encapsulated plant cells. We have used super-resolution and semi-quantitative live-cell imaging in combination with pharmacological,(More)
Growth and development are coordinated by an array of intercellular communications. Known plant signaling molecules include phytohormones and hormone peptides. Although both classes can be implicated in the same developmental processes, little is known about the interplay between phytohormone action and peptide signaling within the cellular(More)
Formal verification methods have gained increased importance due to their ability to guarantee system correctness and improve reliability. Nevertheless, the question how proofs are to be formalized in theorem provers is far from being trivial, yet very important as one needs to spend much more time on verification if the formal-ization was not cleverly(More)
Model-driven software development (MDD) is seen as a promising approach to improve software quality and reduce production costs significantly. However, one of the problems in using MDD especially in the area of safety-critical systems is the lack of verified transformations. The verification of crucial safety properties on the model level is only really(More)
Cross-talk between plant cells and their surroundings requires tight regulation of information exchange at the plasma membrane (PM), which involves dynamic adjustments of PM protein localization and turnover to modulate signal perception and solute transport at the interface between cells and their surroundings. In animals and fungi, turnover of PM proteins(More)
INTRODUCTION The possible role of UCP2 in modulating mitochondrial Ca2+-uptake (mCa2+-uptake) via the mitochondrial calcium uniporter (MCU) is highly controversial. METHODS Thus, we analyzed mCa2+-uptake in isolated cardiac mitochondria, MCU single-channel activity in cardiac mitoplasts, dual Ca2+-transients from mitochondrial ((Ca2+)m) and intracellular(More)
Correctness of compilers is a vital precondition for the correctness of the software translated by them. In this paper, we present two approaches for the formaliza-tion of static single assignment (SSA) form together with two corresponding formal proofs in the Isabelle/HOL system, each showing the correctness of code generation. Our comparison between the(More)
UML specifications offer the advantage to describe software systems while the actual task of implementing code for them is passed to code generators that automatically produce e.g. Java code. For safety reasons, it is necessary that the generated code is semantically equivalent to the original UML specification. In this paper, we present our approach to(More)