Johanna Buchert

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Plate assays for lipoxygenase producing microorganisms on agar plates have been developed. Both potassium iodide-starch and indamine dye formation methods were effective for detecting soybean lipoxygenase activity on agar plates. A positive result was also achieved using the β-carotene bleaching method, but the sensitivity of this method was lower than the(More)
BACKGROUND Sulfhydryl oxidases are flavin-dependent enzymes that catalyse the formation of de novo disulfide bonds from free thiol groups, with the reduction of molecular oxygen to hydrogen peroxide. Sulfhydryl oxidases have been investigated in the food industry to remove the burnt flavour of ultraheat-treated milk and are currently studied as potential(More)
Sulfhydryl oxidases (SOX) are FAD-dependent enzymes capable of oxidising free thiol groups and forming disulphide bonds. Although the quantity of scientific papers and suggested applications for SOX is constantly increasing, only a limited number of microbial SOX have been reported and are commercially available. Hence, the aim of this study was to develop(More)
  • Chiara Gasparetti, Juha Kallio, Nina Janne Hakulinen, Juha Jänis, Msc Heidi Rouvinen, Kaljunen +20 others
  • 2012
Biochemical and structural characterisation of the copper containing oxidoreductases catechol oxidase, tyrosinase, and laccase from ascomycete fungi are copper-containing metalloenzymes. They oxidise substituted phenols and use molecular oxygen as a terminal electron acceptor. Catechol oxidases and tyrosinases catalyse the oxidation of p-substituted(More)
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