The classic molecular biology methods like Northern or Southern blot analyse non-amplified DNA or RNA, but need large amounts of nucleic acids, in many instances from tissues or cells that are heterogeneous. In contrast, polymerase chain reaction (PCR)-based techniques allow us to obtain genetic information through the specific amplification of nucleic acid… (More)
Myasthenia gravis (MG) patients develop autoantibodies primarily against the acetylcholine receptor in the motor endplate, but also against intracellular striated muscle proteins, notably titin, the giant elastic protein of the myofibrillar cytoskeleton. Titin antibodies have previously been shown to be directed against a single epitope on the molecule,… (More)
Wilhelm Hasselbach (1921–2015) is best known for his major contribution to the discovery and molecular elucidation of an ATP-driven active ion transport across a biological membrane. He had discovered SERCA, the calcium pump of the sarcoplasmic reticulum (Hasselbach and Makinose in Biochem Z 333:518–528, 1961; Biochem Z 339:94–111, 1963).
Certain psychosomatic disorders such as chronic psychosomatic pain, phobias and other anxiety disorders as well as depression are often stress-related but may also be acquired by learning associated with traumatic experience. As learning is based on changes in neuronal networks, the brain will be altered in these diseases. In turn, brain structure and… (More)
Isometric force responses following flash photolysis of caged-ATP were measured from skinned preparations of the catch muscle anterior byssus retractor of Mytilus (ABRM). When fibres were transferred from Ca(2+)-free to Ca(2+)-containing rigor solution (pCa < 4) the force remained low, but flash photolysis produced an extended force increase (half-time,… (More)
At neutral pH, fully Ca2+ -activated glycerinated dorsal longitudinal fibre bundles from Lethocerus indicus contract under isometric conditions and respond to release by deactivation, i.e. quick release causes a delayed tension fall. At slightly alkaline pH, the release-induced deactivation becomes a transient phenomenon, i.e. a delayed tension fall is… (More)
Bundles of glycerol-extracted psoas fibres which were contracted by immersion in a saline containing 15 mM MgATP and 12 micron free Ca++ were subjected to up to 3 stretches (rise time 0.8 msec) each of amplitude 1% Li at intervals of 10 msec. The elastic tension responses to these stretches were all of comparable size and the peak tensions reached during… (More)