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Automated molecular microscopy: the new Leginon system.
TLDR
We report here on the current state of our efforts in automated molecular microscopy. Expand
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A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron
Molecular self-assembly offers a means of spontaneously forming complex and well-defined structures from simple components. The specific bonding between DNA base pairs has been used in this way toExpand
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Supramolecular Architecture of Severe Acute Respiratory Syndrome Coronavirus Revealed by Electron Cryomicroscopy
ABSTRACT Coronavirus particles are enveloped and pleomorphic and are thus refractory to crystallization and symmetry-assisted reconstruction. A novel methodology of single-particle image analysis wasExpand
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Automated cryoEM data acquisition and analysis of 284742 particles of GroEL.
TLDR
We demonstrate the potential of the Leginon system for high-throughput data acquisition by describing an experiment in which we acquired images of more than 280,000 particles of GroEL in a single 25 h session at the microscope. Expand
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A toolbox for ab initio 3-D reconstructions in single-particle electron microscopy.
TLDR
We have developed a "toolbox" of ab initio reconstruction techniques that provide several options for calculating 3-D volumes in an easily managed and tightly controlled work-flow that adheres to standard conventions and formats. Expand
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Towards automated screening of two-dimensional crystals.
TLDR
We have developed working prototypes for several of these tools and tested and evaluated them in a simple screen of 24 crystallization conditions. Expand
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Unnatural amino acid incorporation into virus-like particles.
Virus-like particles composed of hepatitis B virus (HBV) or bacteriophage Qbeta capsid proteins have been labeled with azide- or alkyne-containing unnatural amino acids by expression in a methionineExpand
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Effects of Metronidazole, Tetracycline, and Bismuth-Metronidazole-Tetracycline Triple Therapy in the Helicobacter pylori SS1 Mouse Model after 1 Day of Dosing: Development of an H. pylori Lead
ABSTRACT We evaluated the effect of optimized doses and dosing schedules of metronidazole, tetracycline, and bismuth-metronidazole-tetracycline (BMT) triple therapy with only 1 day of dosing onExpand
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Ultrastructure of SARS-CoV, FIPV, and MHV Revealed by Electron Cryomicroscopy
* The Scripps Research Institute, La Jolla, California. The current understanding of coronavirus ultrastructure relies heavily on transmission electron microscopy of negatively stained images. SuchExpand
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Automation in single-particle electron microscopy connecting the pieces.
TLDR
We provide a generic definition of and discuss some of the most important advances in automated approaches to specimen preparation, grid handling, robotic screening, data acquisition, image processing, and computational infrastructure. Expand
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