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The ability to induce targeted mutations in somatic cells in developing organisms and then track the fates of those cells is a powerful approach both for studying neural development and for modeling human disease. The CRISPR/Cas9 system allows for such targeted mutagenesis, and we therefore tested it in combination with a piggyBac transposase lineage(More)
The relatively noise free images obtained from the cyrosection dataset of the Visible Woman simplifies the automation of the extraction of vascular and other fluid filled anatomical structures. However, the complex textures associated with other tissues within organs make the automatic extraction of organs based solely on imaging techniques challenging [1].(More)
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