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Understanding the origins and roles of cardiac progenitor cells is important for elucidating the pathogenesis of congenital and acquired heart diseases. Moreover, manipulation of cardiac myocyte progenitors has potential for cell-based repair strategies for various myocardial disorders. Here we report the identification in mouse of a previously unknown(More)
Compartmentation and dynamics of cAMP and PKA signaling are important determinants of specificity among cAMP's myriad cellular roles. Both cardiac inotropy and the progression of heart disease are affected by spatiotemporal variations in cAMP/PKA signaling, yet the dynamic patterns of PKA-mediated phosphorylation that influence differential responses to(More)
Seventy-nine Sprague-Dawley derived primimparous rats were injected subcutaneously throughout pregnancy and the nursing period with either (1) 30 mg/kg of pure nicotine, (2) 5.0 mg/kg methamphetamine HCL, (3) saline vehicle, or, (4) non-injected. Vital and developmental measures were taken on the offspring throughout the nursing period and for one(More)
An unselected sample of 1529 women (predominantly white, married, and middle-class) were interviewed during pregnancy regarding their use of alcohol, nicotine, caffeine, drugs, and other variables. Subsets of offspring were examined to assess the relationship of self-reported maternal alcohol use to infant health and development. Multiple regression(More)
The teratogenicity of alcohol has been demonstrated in humans through clinical studies, behavioral studies, and epidemiologic studies, and in animals through controlled laboratory experiments. In humans exposed to alcohol during gestation the effects can range from fetal alcohol syndrome in some offspring of chronic alcoholic women to reduced average birth(More)
This substudy of a longitudinal prospective study was designed to assess neurodevelopmental and neurobehavioral performance in newborn infants who were maternally exposed to cocaine and other drugs of abuse or the other drugs without cocaine. Sample selection procedures were designed to permit statistical control for marijuana, alcohol, and tobacco. Cocaine(More)
Fluorescence lifetimes of single Rhodamine 6G molecules on silica surfaces were measured with pulsed laser excitation, time-correlated single photon counting, and near-field scanning optical microscopy (NSOM). The fluorescence lifetime varies with the position of a molecule relative to a near-field probe. Qualitative features of lifetime decreases are(More)
Twenty-five Sprague-Dawley derived rats were administered 1.0, 3.0 or 5.0 mg/kg of methamphetamine HC1 or saline twice daily throughout gestation beginning on Day 1 of pregnancy. Rats were allowed to deliver normally; offspring were culled to 8 and sexed on Day 7, and weaned on Day 21. All females had viable litters except at the 5.0 mg/kg dose where 4 of 7(More)
The measure of time was used as an additional parameter on an existing flow cytometer to study the kinetics of enzyme activities and cell-stain interactions. By correlating all fluorescent signals from single cells with time, the dynamics of a reaction can be followed for several minutes. This advanced application of flow cytometry is easily implemented and(More)
A modified flow cytometer has been used to detect attogram quantities of aqueous rhodamine 6G by laser-induced fluorescence analysis. A detection limit of 28 attograms (35,000 molecules) was obtained, nearly two orders of magnitude better than earlier measurements. The detection limit in concentration units was 1.4 x 10(-13) mole per liter. During the(More)