Joanna Pyczek

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Pluripotent stem cells have the therapeutic potential in future regenerative medicine applications. Therefore, it is highly important to understand the molecular mechanisms governing the pluripotency and differentiation potential of these cells. Our current knowledge of pluripotent cells is largely limited owing to the candidate gene/protein approach rather(More)
Hedgehog (HH) signaling is known to be essential during the embryonal development of the pituitary gland but the knowledge about its role in the adult pituitary and in associated tumors is sparse. In this report we investigated the effect of excess Hh signaling activation in murine pituitary explants and analyzed the HH signaling status of human(More)
The use of feeder based protocols for the creation, expansion and banking of hES cell lines are well established. The advances in technology for feeder free culture have predominantly relied on the cultivation of pluripotent cells in colony based systems, commonly used in conjunction with ill-defined matrices. We have developed a process to allow enzymatic(More)
BACKGROUND Rhabdomyosarcoma (RMS) is the most common type of soft tissue sarcoma in children. The Hedgehog (HH) pathway is known to develop an oncogenic role in RMS. However, the molecular mechanism that drives activation of the pathway in RMS is not well understood. METHODS The expression of HH ligands was studied by qPCR, western blot and(More)
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