We hypothesized that the mechanically active environment present in rotating bioreactors mediates the effectiveness of three-dimensional (3D) scaffolds for cartilage tissue engineering. Cartilaginous constructs were engineered by using bovine calf chondrocytes in conjunction with two scaffold materials (SM) (benzylated hyaluronan and polyglycolic acid);… (More)
Spinefreie GABAergic neurons in the visual cortex of the rat were studied with the Golgi-Kopsch-method and immunohistochemical methods against GABA. They show differences of their axonal arborizations, axonal surfaces and of their axonal terminal formations. We found large spinefree neurons, spinefree neurons with strictly axonal fields, spinefree neurons… (More)
To demonstrate the DFP-effects on the AChE of the mesencephalic raphe region albino rats were investigated using the pharmaco-histochemical method of Butcher et al. (1975). At several times after 1 mg DFP/kg body weight, the histochemical reactions in certain AChE-containing neurons were measured semiquantitatively and compared with biochemical data.… (More)
The influence of alpha- and beta-receptor antagonists combined with drugs which act as antagonists at muscarinic and nicotinic receptor sites on epileptic focus produced by topic penicillin application was investigated. The beta-receptor blocking agent caused a faster and more intense activity of epileptogenic focus in comparison with activity in control… (More)
In albino rats, the nucleus raphes dorsalis (NRD) of the midbrain was investigated using a sensitive fluorochroming procedure to detect indolamines in combination with a microelectrophoretic method to demonstrate the isoenzyme pattern of acetylcholinesterase (AChE). Based on the fluorescence histochemical appearance, 3 types of indolaminergic cells were… (More)
The topohistochemistry of gamma-aminobutyric acid (GABA) was studied in the hippocampus of rats with epileptiform activity provoked by cortical implantation of cobalt-agar pellets and in control rats with implanted agar-pellets. Using the GABA specific fluorochroming procedure of Wolman (1971) a distinct decrease of GABA fluorescence was found especially in… (More)
Stimulation of the nucleus raphe dorsalis (B7) with low frequency electrical current in freely moving albino rats provoked an increase of hypersynchronous activity in the epileptogenic focus. The time course of this effect points to an action of the liberated 5-hydroxytryptamine.
After selective application of horseradish peroxidase (HRP) into the dorsal part of the lateral geniculate nucleus (LGN) of the albino rat, cells were labelled through the retrograde transport of the enzyme in the following nuclei of the brain stem: 1. Ncl. raphes linearis, 2. Nel, raphes centralis, 3. Ncl. raphes dorsalis, 4. Ncl. ventralis rostralis… (More)