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1. The subcellular characteristics of an ATP-induced elevation of the cytoplasmic free calcium concentration ([Ca2+]i) were studied in cultured cells of the oligodendrocyte lineage obtained from mouse cortex and rabbit retina, as well as in oligodendrocytes from mouse corpus callosum slices, using laser scanning confocal microfluorometry. 2. With the stage-(More)
We studied the distribution of voltage-gated Ca2+ channels in cells of the oligodendrocyte lineage from retinal and cortical cultures. Influx of Ca2+ via voltage-gated channels was activated by membrane depolarization with elevated extracellular K+ concentration ([K+]e) and local, subcellular increases in cytosolic free Ca2+ concentration ([Ca2+]in) could(More)
The response of retinal microglial cells, which accompanies retrograde degeneration of ganglion cell axons and perikarya (induced by transection of the optic nerve), was studied in whole-mounted rabbit retinae labeled enzyme-histochemically for nucleoside diphosphatase (NDPase), which is a microglial cell marker. A few days after transection, the number of(More)
Monolayer cultures were prepared from two distinct parts of early postnatal rabbit retinae. Cell suspensions obtained from the developing medullary ray (MR) region contained neurons, Müller (glial) cells, and astrocytes, cells obtained from the remainder (peripheral) part of the retina contained neurons and Müller cells, but no astrocytes. Müller cells lack(More)
Rabbit retinae were stained with antibodies to glial fibrillary acidic protein (GFAP) at various times up to 5 months after complete unilateral intraorbital optic nerve transection, which is known to induce degeneration of ganglion cell axons and perikarya in the retina. A transient immunoreactivity for GFAP was observed in Müller glial cells that normally(More)
Vascularized mammalian retinae contain two distinct neuroglial cells types, radially oriented Müller cells and astrocytes, which are located in the nerve fiber layer. These cell types derive from different precursor cells and proliferate during ontogenesis at distinct schedules. The aim of the present study was to disclose whether growth factors, which are(More)
Physical plasmas which contain a mixture of different radicals, charged species and UV-radiation, have recently found entry in various medical applications. Though first clinical trials are underway nothing is known about the plasma components mediating the biological effects seen and safety concerns have been neglected. We here use for the first time a(More)
The differentiation of oligodendrocytes from undifferentiated progenitor cells was studied in cultures obtained from the postnatal rabbit retina. 'Sandwich' cultures were established by turning the coverslips with adhering cells up-side down about 24 h after seeding. As a result O4-positive oligodendrocyte progenitors stop dividing and differentiate. Within(More)
Ultrastructural studies of the liver from a non-alcoholic 21 year old male with focal nodular hyperplasia (FNH) showed hepatocyte cytoplasmic alterations including swollen and bizarre mitochondria, rough endoplasmic reticulum (RER) in short parallel stacks or whorl-like arrangements, and Mallory bodies (MB). Proliferated bile duct cells contained occasional(More)