João F. P. Crespo

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The main objective of the present study was to investigate the use of in situ 2D fluorometry for monitoring key bioprocess variables in mammalian cell cultures, namely the concentration of viable cells and the concentration of recombinant proteins. All studies were conducted using a recombinant Baby Hamster Kidney (BHK) cell line expressing a fusion(More)
Pastures are assumed to be good sources of alpha-linolenic acid (ALA) and other bioactive compounds. In this study, we evaluated the effects of restricting the intake of a cereal-based feed on the consumption of a legume-based pasture, and consequently on poultry performance and meat quality. Broilers of the RedBro Cou Nu x RedBro M genotype were fed a(More)
Free-range chickens are assumed to consume low to moderate levels of pasture, although the effects of forage intake in broiler performance and poultry meat quality remain to be established. In addition, despite cellulases and hemicellulases being widely used as feed supplements to improve the nutritive value of cereal-based diets for fast-growing broilers,(More)
Dehydrated forages are assumed to be good sources of alpha-linolenic acid (ALA) and lipid-soluble antioxidant compounds (vitamin E homologs and beta-carotene). The effects of including a dehydrated leguminous-based forage in a typical diet for broiler chicken, on performance, meat quality, and fatty acid composition were evaluated. One hundred sixty 1-d-old(More)
A mathematical model of steady state reactor behavior was developed, combining the tanks-in-series model with fermentation kinetic description. The model was tested with experimental data-from an immobilized cell reactor for propionic acid production operated under different residence times. In order to determine the effects of liquid flow rate and gas(More)
When comparing 2-D shapes, a key issue is their normalization. Translation and scale are easily taken care of by removing the mean and normalizing the energy. However, defining and computing the orientation of a 2-D shape is not so simple. In fact, although for elongated shapes the principal axis can be used to define one of two possible orientations, there(More)
The analytic signature is a recently proposed 2D shape representation scheme. It is tailored to the representation of shapes described by arbitrary sets of unlabeled points, or landmarks, because its most distinctive feature is the maximal invariance to a permutation of those points. The shape similarity of two point clouds can then be obtained from a(More)
There is an ever-increasing demand for high quality forage legumes, essential to feed livestock. Micropropagation may be useful to preserve elite allelic compositions in allogamous and auto-incompatible forage legume species, as is the case of Trifolium resupinatum L. (Persian clover). Etiolation-like phenotypes in in vitro cultured plants, with long weak(More)
When comparing 2D shapes, a key issue is their normalization. Translation and scale are easily taken care of by removing the mean and normalizing the energy. However, defining and computing the orientation of a 2D shape is not so simple. In fact, although for elongated shapes the principal axis can be used to define one of two possible orientations, there(More)
1. The supplementation of diets rich in soluble polysaccharides with microbial cellulases and hemicellulases decreases digesta viscosity and promotes broiler performance. 2. In contrast, recent experiments suggest that polysaccharidases are ineffective for improving the nutritive value of pasture biomass used by free-range broilers. However, the feasibility(More)
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