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Interferon-gamma when given intraperitoneally by single daily injection into one-day-old rats upregulated the expression of major histocompatibility complex class I antigen on ramified microglial cells in cerebral cortex and induced the expression of major histocompatibility complex class II antigen both on amoeboid and ramified microglial cells present in(More)
We have attempted to clarify the mechanisms for alcohol (EtOH)-induced elevation of diazepam binding inhibitor (DBI) mRNA and to investigate whether the increase in DBI mRNA is paralleled with that in DBI using EtOH-treated mice and primary cultured neurons. Both the DBI content and the expression of DBI mRNA were elevated in the cerebral cortex of(More)
The effects of bacterial lipopolysaccharide (LPS) on the expression of surface antigens including major histocompatibility complex (MHC) and complement type 3 (CR3) receptors on microglial cells in the corpus callosum in postnatal rat brain were investigated. When LPS was injected intravenously (i.v.) in 1-d-old rats, the immunostaining of callosal amoeboid(More)
The ultrastructure of the capillaries and their permeability to lanthanum ions and ferritin in the corpus callosum was examined in postnatal rat brain. In 1 and 7-d-old rats, numerous pinocytotic vesicles were observed in the endothelial cytoplasm of the callosal capillaries. Tight junctions were present between adjacent endothelial cells which were(More)
Amoeboid microglial cells (AMC) in the corpus callosum were selectively labelled following a single intraperitoneal (i.p.) injection of the fluorescent dye, rhodamine isothiocyanate (RhIc) into postnatal rats. The frequency of RhIc-labelled cells varied with age, with the largest number occurring in 7-d-old animals. Thereafter, the labelled cells declined(More)
When HRP was injected intraperitoneally (i. p.), labelled amoeboid microglial cells (AMC) were consistently localized in the subcortical white matter and circumventricular zones in early postnatal (1 and 7 days old) but absent in late postnatal (14-day-old) rats. The ingested HRP disappeared from the labelled cells 5 days after IP injection. Subcutaneous(More)
Several lines of evidence indicate that phosphatidylinositol 3-kinase (PI3K)-protein kinase B/Akt (PKB/Akt) signal pathway mediate the pain hypersensitivity induced by intradermal injection of capsaicin, and nerve growth factor induced upregulation of vanilloid receptor 1 in dorsal root ganglia (DRG) neuron via the activation of PI3K. While, the roles of(More)
The induction of major histocompatibility complex (MHC) class II antigen on amoeboid microglia cells (AMC) by lipopolysaccharide (LPS) and interferon-gamma (INF-gamma) in early postnatal rat brain was studied by immunohistochemistry. In newborn rats given successive intraperitoneal injections of LPS or INF-gamma and killed at the age of 7 days, MHC class II(More)
Effect of chronic treatment with nicotine on DBI and its mRNA in mouse cerebral cortex were examined. Continuous treatment of mice with nicotine significantly increased DBI content and its mRNA expression, which was completely abolished by simultaneous administration of mecamylamine (1 mg/kg, i.p.). These results indicate that chronic functional interaction(More)
We investigated the effects of nitric oxide (NO) on voltage-dependent Ca2+ channels (VDCCs) by examining [45Ca2+]influx into mouse cerebral cortical neurons. S-nitroso-N-acetylpenicillamine (SNAP) induced a dose-dependent increase in [45Ca2+]influx, which was completely abolished by hemoglobin, tetrodotoxin and dibucaine. The NO-induced [45Ca2+influx was(More)