Jiri Krivanek

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Activity of Na, K-ATPase in homogenates of fresh cerebral cortex of rats was compared with that of cortex frozen under different conditions. Activity yields after rapid in situ freezing of the exposed cerebral cortex were twice, higher (26.1 U) than in homogenates of the fresh cortex (13.3 U). Fresh brain kept on ice for 60 and 300 s and subsequently frozen(More)
Activity and distribution of protein kinase C (PKC) in the rat cerebral cortex was correlated with the development of spreading depression. When the 'waves' of the slow potential shift, induced by topical application of concentrated KCl solutions, were allowed to spread over the cerebral cortex for 10 min, both cytosolic and particulate fractions of the(More)
The enzymatic activity of protein kinase C (PKC) was measured in the cytosol and particulate fraction of parabrachial nucleus, the presumed site of conditioned taste aversion (CTA) engrams. At various time intervals after acquisition of the task (pairing saccharin consumption with subsequent LiCl poisoning) the nucleus was dissected from the frozen coronal(More)
A method for the dissection of the nucleus parabrachialis (NPB) from the coronal sections of frozen rat brain is described. The protein kinase C (PKC) activity was determined in the cytosol and particulate fractions of the pooled samples of the nucleus. The effect of the i.p. administration of the conditioned taste aversion-inducing agents LiCl and CuSO4 on(More)
D-Amphetamine (AM) is a potent inducer of conditioned taste aversion (CTA) the mechanism of which differs from that induced by lithium. The aim of the present communication is to see whether AM-induced CTA will produce shift in the protein kinase (PKC) activity in the parabrachial nucleus (PBN). Activity of PKC was measured in PBN of rats during AM-induced(More)
Repetitive generation of spreading depression (SD) waves may induce metabolic and functional disturbances in the invaded brain regions. In order to better characterize this state, up to 60 SD waves were elicited in the cerebral cortex of anesthetized rats by intracortical injections of 2 microliters of isotonic K-acetate repeated at regular 5-min intervals.(More)
The contribution of protein kinase C (PKC) to the acquisition of conditioned taste aversion (CTA) was tested by injection of three PKC inhibitors--polymyxin B, H7 and staurosporine--into the parabrachial nucleus (PBN). From the tested drugs only polymyxin B (20 mM) prevented CTA acquisition. Application of H7 (10 mM) and staurosporine (100 and 500 microM)(More)
Cyclic AMP (cAMP) was determined in that part of the rat cerebral cortex which was invaded by slow potential change (SPC) accompanying cortical spreading depression (CSD). cAMP was determined at various phases of SPC development and at several time intervals after SPC recovery. At maximum SPC, cAMP was increased by 100% above control and by 116% at the time(More)
Bielavska and colleagues (Bielavska, Sacchetti, Baldi, & Tassoni, 1999) have recently shown that KN-62, an inhibitor of calcium/calmodulin-dependent kinase II (CaCMK), induces conditioned taste aversion (CTA) when introduced into the parabrachial nucleus (PBN) of rats. The aim of the present report was to assess whether activity of CaCMK in the PBN is(More)