Jiri Janata

Zdenek Kamenik4
Lucie Najmanova3
Jitka Novotna3
Radek Gazak3
Marketa Koberska2
4Zdenek Kamenik
3Lucie Najmanova
3Jitka Novotna
3Radek Gazak
2Marketa Koberska
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We report on a CMOS-based microsensor array, featuring 576 electrodes and 24 channels, for label-free electrochemical detection of DNA hybridization. Each channel comprises a potentiostatic circuit that keeps the electrode at a defined potential and measures the respective electrical current. The readout channels rely on a first-order delta-sigma(More)
  • Petra Jiraskova, Radek Gazak, Zdenek Kamenik, Lucie Steiningerova, Lucie Najmanova, Stanislav Kadlcik +3 others
  • 2016
Structurally different and functionally diverse natural compounds - antitumour agents pyrrolo[1,4]benzodiazepines, bacterial hormone hormaomycin, and lincosamide antibiotic lincomycin - share a common building unit, 4-alkyl-L-proline derivative (APD). APDs arise from L-tyrosine through a special biosynthetic pathway. Its generally accepted scheme, however,(More)
  • Jiri Janata, Stanislav Kadlcik, Marketa Koberska, Dana Ulanova, Zdenek Kamenik, Petr Novak +6 others
  • 2015
In the biosynthesis of lincosamide antibiotics lincomycin and celesticetin, the amino acid and amino sugar units are linked by an amide bond. The respective condensing enzyme lincosamide synthetase (LS) is expected to be an unusual system combining nonribosomal peptide synthetase (NRPS) components with so far unknown amino sugar related activities. The(More)
  • Lucie Najmanova, Dana Ulanova, Marketa Jelinkova, Zdenek Kamenik, Eliska Kettnerova, Marketa Koberska +3 others
  • 2014
The biosynthetic gene cluster of porothramycin, a sequence-selective DNA alkylating compound, was identified in the genome of producing strain Streptomyces albus subsp. albus (ATCC 39897) and sequentially characterized. A 39.7 kb long DNA region contains 27 putative genes, 18 of them revealing high similarity with homologous genes from biosynthetic gene(More)
The gene lmbB2 of the lincomycin biosynthetic gene cluster of Streptomyces lincolnensis ATCC 25466 was shown to code for an unusual tyrosine hydroxylating enzyme involved in the biosynthetic pathway of this clinically important antibiotic. LmbB2 was expressed in Escherichia coli, purified near to homogeneity and shown to convert tyrosine to(More)
Eukaryotic cells begin to assemble discrete, nucleoplasmic repair foci within seconds after the onset of exposure to ionizing radiation. Real-time imaging of this assembly has the potential to further our understanding of the effects of medical and environmental radiation exposure. Here, we describe a microirradiation system for targeted delivery of(More)
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