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The ACC deaminase gene (acdS) from Enterobacter cloacae UW4 was replaced by homologous recombination with the acdS gene with a tetracycline resistance gene inserted within the coding region. Upon characterization of this AcdS minus mutant, it was determined that both ACC deaminase activity and the ability to promote the elongation of canola roots under(More)
OBJECTIVE To investigate microstructural tissue changes of trigeminal nerve (TGN) in patients with unilateral trigeminal neuralgia (TN) by multiple diffusion metrics, and correlate the diffusion indexes with the clinical variables. METHODS 16 patients with TN and 6 healthy controls (HC) were recruited into our study. All participants were imaged with a(More)
The present study was conducted to investigate the prevalence and genotypes of Cryptosporidium in bats. A total of 247 bats, belonging to Rhinolophus sinicus , Rousettus leschenaultia, Aselliscus stoliczkanus , and Hipposideros fulvus , were collected in Yunnan Province, Southwestern China, and the intestinal tissues were examined for Cryptosporidium(More)
BACKGROUND Although self-management reportedly can improve the health and quality of life of patients with COPD, there is no validated instrument for evaluating the status of the self-management of patients with COPD. METHODS A 51-item COPD Self-Management Scale (CSMS), including 5 domains (symptom management, daily life management, emotion management,(More)
This purpose was to discuss optimal reference value for normal glucose tolerance (NGT) at different time points during an oral glucose tolerance test (OGTT) by receiver operating characteristic (ROC) analysis. One thousand seven hundred and forty-six subjects from March 2004 to March 2005 were given 75 g OGTT and the blood samples were collected at 0, 30,(More)
A new antimicrobial peptide (AMP) named PGLa-H has been isolated from the skin of the African clawed frog (Xenopus laevis) using gel filtration and reverse-phase high-performance liquid chromatography (RP-HPLC). Its amino acid sequence was determined as KIAKVALKAL by Edman degradation, with a molecular weight of 1053.727 Da as analysed by matrix-assisted(More)
We detected Toxoplasma gondii in 29.3% (95% confidence interval [CI], 25.5% to 33.1%) of 550 insectivorous bats collected in Myanmar. The genotyping of these positive samples revealed they were closely related to or belong to clonal type I, which is highly virulent in mice, showing that these bats are potential reservoirs for T. gondii transmission.
The precise detection of Toxoplasma gondii infection in cats has important public health significance. In the present study, recombinant granule antigen protein GRA7 was evaluated as a potential diagnostic marker for T. gondii infection in cats by an indirect enzyme-linked immunosorbent assay (ELISA), using the classified serum samples from cats, by(More)
Several single nucleotide polymorphisms (SNPs) of the Glutamate metabotrophic receptor 7 gene (GRM7) have recently been identified by the genome-wide association study (GWAS) as potentially playing a role in susceptibility to age-related hearing impairment (ARHI), however this has not been validated in the Han Chinese population. The aim of this study was(More)
Following transposon Tn5 mutagenesis of the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2, mutants that have different freeze-resistant properties were selected. Five of the freeze-sensitive mutants, i.e. FSM-5, -6, -14, -29, and -41, secreted a lower amount of antifreeze protein-(AFP) into the culture broth compared with the wild-type.(More)