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The solventogenic bacterium Clostridium acetobutylicum is an important species of the Clostridium community. To develop a fundamental tool that is useful for biological studies of C. acetobutylicum, we established a high resolution proteome reference map for this species. We identified 1206 spots representing 564 different proteins by mass spectrometry,(More)
Different geometries of nitromethane dimer and nitromethane trimer have been fully optimized employing the density functional theory B3LYP method and the 6-31++G** basis set. Three-body interaction energy has been obtained with the ab initio supermolecular approach at the levels of MP2/6-31++G**//B3LYP/6-31++G** and MP2/aug-cc-pVDZ//B3LYP/6-31++G**. The(More)
Paenibacillus polymyxa can produce the (R,R)-stereoisomer of 2,3-butanediol (2,3-BDL) which is industrially very useful. Two important factors affecting (R,R)-BDL production by P. polymyxa ATCC 12321, medium composition, and addition of acetic acid to the culture were investigated in this study with accompanying comparative proteomic analysis. For this(More)
Grounded on linear ordinary differential formulation, we propose an optimization model for inferring gene regulatory networks, which integrates with not only the sparsity principle of gene networks but also some extra priori knowledge between two genes that can be found in existing publications or biological web sites. The model is applied to an artificial(More)
Dimension problem is the main difficulty in inferring gene regulatory networks and has not been solved in substance. Grounded on linear ordinary differential equation, we propose a simple optimization model for achieving sparseness for the derived gene regulatory networks. The model is applied to gene expression profile data related to breast cancer(More)
ManH, a novel substrate-binding protein of an ABC transporter, was identified from the mannan utilization gene cluster of Bacillus sp. N16-5. We cloned, overexpressed, and purified ManH and measured its binding affinity to different substrates by isothermal titration calorimetry. ManH binds to mannotriose, mannotetraose, mannopentose, and(More)
The RDX single crystals are ignited by ultraviolet laser (355 nm, 6.4 ns) pulses. The laser-induced damage morphology consisted of two distinct regions: a core region of layered fracture and a peripheral region of stripped material surrounding the core. As laser fluence increases, the area of the whole crack region increases all the way, while both the area(More)
Wind power prediction is of great importance for the safety and stabilization of grids. The most important and difficult problem now is to enhance the prediction precision. A combined wind power prediction model based on the maximum information entropy principle was built in this paper. The wind power series is non-gauss distribution, so the prediction(More)
Density functional theory and volume-based thermodynamics calculations were performed to study the effects of different substituents and linkages on the densities, heats of formation (HOFs), energetic properties, and thermodynamics of formation for a series of energetic nitrogen-rich salts composed of triaminoguanidinium cation and 5-nitroiminotetrazolate(More)
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