Jingjing Cuan

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We compared results obtained from two parathyrin (parathyroid hormone) assays with differing specificities, using sera from 172 normal donors and from 98 patients with disorders of calcium regulation. Intact parathyrin was measured in both assays; the C-parathyrin assay also measured the 53-84 amino acid C-terminal hormone fragment; the N-parathyrin assay(More)
BACKGROUND AND OBJECTIVES The diagnosis of mucocutaneous herpes simplex virus (HSV) is hampered by suboptimal sensitivity of virus culture and atypical clinical morphology. GOALS To compare the diagnostic usefulness of the polymerase chain reaction (PCR) and virus culture. STUDY DESIGN Consecutive samples from 246 patients at an urban sexually(More)
We separated the hemoglobins in an unwashed erythrocyte hemolysate according to their isoelectric points on a thin-layer horizontal polyacrylamide gel containing ampholyte (40 g/L) over a pH gradient of 6-8. We scanned the fixed, unstained gels by microdensitometry and calculated the percentage of hemoglobin A1C. The overall CV (between-run imprecision) for(More)
We describe a sequential double-antibody assay for measuring C-terminal parathyrin in serum with commercially available reagents. Intact bovine hormone, used as a working standard, is iodinated by conventional Chloramine T procedures. The antibody affinity is characteristic of high affinity binding (1.4 to 1.6 x 10(10) L/mol of intact parathyrin). The(More)
We subcloned six discrete protein-coding regions representing the gag (Kp24 and Kp55), env (Kp41, Kp120N, and Kp120CC), and pol (Kp66/31) gene products of the human immunodeficiency virus type 1 (HIV-1) and expressed them in Escherichia coli as fusion proteins with the first 56 residues of galactokinase. An enzyme-linked immunosorbent assay for confirming(More)
This study was aimed at understanding the histopathologic changes that occur in the nasal mucosa of patients with perennial allergic rhinitis after high-intensity focused ultrasound (HIFU) treatment. Biopsy specimens of the inferior turbinate mucosa were taken from 11 PAR patients before, immediately after and 1 y after HIFU treatment. Morphometric analysis(More)
OBJECTIVE To present a brief review of the diagnostic benefits of quantitating viral load for hepatitis C and how the reverse transcriptase polymerase chain reaction is being used as an aid to better diagnose and manage the disease. DATA SOURCE Research articles about hepatitis C and the reverse transcriptase polymerase chain reaction, as well as data(More)
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