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RNA-Seq, a method using next generation sequencing technologies to sequence the transcriptome, facilitates genome-wide analysis of splice junction sites. In this paper, we introduce SOAPsplice, a robust tool to detect splice junctions using RNA-Seq data without using any information of known splice junctions. SOAPsplice uses a novel two-step approach(More)
To date, occurrence and stimulation of different nitrification pathways in acidic soils remains unclear. Laboratory incubation experiments, using the acetylene inhibition and 15N tracing methods, were conducted to study the relative importance of heterotrophic and autotrophic nitrification in two acid soils (arable (AR) and coniferous forest) in subtropical(More)
We study the problem of large-scale social identity linkage across different social media platforms, which is of critical importance to business intelligence by gaining from social data a deeper understanding and more accurate profiling of users. This paper proposes HYDRA, a solution framework which consists of three key steps: (I) modeling heterogeneous(More)
Colobines are a unique group of Old World monkeys that principally eat leaves and seeds rather than fruits and insects. We report the sequencing at 146× coverage, de novo assembly and analyses of the genome of a male golden snub-nosed monkey (Rhinopithecus roxellana) and resequencing at 30× coverage of three related species (Rhinopithecus bieti,(More)
Amending vegetable soils with organic materials is increasingly recommended as an agroecosystems management option to improve soil quality. However, the amounts of NO, N2O, and N2 emissions from vegetable soils treated with organic materials and frequent irrigation are not known. In laboratory-based experiments, soil from a NO 3 − -rich (340 mg N kg−1)(More)
The mechanisms underlying the retention of inorganic N in acidic forest soils in southern China are not well understood. Here, we simultaneously quantified the gross N transformation rates of various subtropical acidic forest soils located in southern China (southern soil) and those of temperate forest soils located in northern China (northern soil). We(More)
The potential denitrification (PD) rate, NO, N2O, and N2 emission were determined after treatment with 50 mg NO3 −−N kg−1 soil using the acetylene inhibition method, and meanwhile abundance of four denitrifying genes (i.e., narG, nirK, norB, nosZ) was also investigated in subtropical soils of China. Soil samples were collected from conifer forest (C), shrub(More)
An in situ 15N tracing study was conducted to investigate the characteristics of soil mineral nitrogen (N) production and conservation in acid forest soils of subtropical China. The six experimental soils were strongly acidic (pH ranged from 4.1 to 4.7), except for one soil, from an orange orchard which had a higher pH (5.7) due to lime application. Total(More)
A laboratory incubation experiment was conducted to investigate the effects of direct incorporation of either wheat straw or its biochar into a cultivated Chernozem on gross N transformations calculated by the 15N pool dilution technique and nitrous oxide (N2O) production rates. Incorporation of wheat straw stimulated gross NH 4 + (ammonium) and NO 3 −(More)