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An in situ 15N tracing study was conducted to investigate the characteristics of soil mineral nitrogen (N) production and conservation in acid forest soils of subtropical China. The six experimental soils were strongly acidic (pH ranged from 4.1 to 4.7), except for one soil, from an orange orchard which had a higher pH (5.7) due to lime application. Total(More)
To date, N(2)O production pathways are poorly understood in the humid subtropical and tropical forest soils. A (15)N-tracing experiment was carried out under controlled laboratory conditions to investigate the processes responsible for N(2)O production in four subtropical acid forest soils (pH<4.5) in China. The results showed that denitrification was the(More)
To date, occurrence and stimulation of different nitrification pathways in acidic soils remains unclear. Laboratory incubation experiments, using the acetylene inhibition and 15N tracing methods, were conducted to study the relative importance of heterotrophic and autotrophic nitrification in two acid soils (arable (AR) and coniferous forest) in subtropical(More)
Amending vegetable soils with organic materials is increasingly recommended as an agroecosystems management option to improve soil quality. However, the amounts of NO, N2O, and N2 emissions from vegetable soils treated with organic materials and frequent irrigation are not known. In laboratory-based experiments, soil from a NO 3 − -rich (340 mg N kg−1)(More)
The potential denitrification (PD) rate, NO, N2O, and N2 emission were determined after treatment with 50 mg NO3 −−N kg−1 soil using the acetylene inhibition method, and meanwhile abundance of four denitrifying genes (i.e., narG, nirK, norB, nosZ) was also investigated in subtropical soils of China. Soil samples were collected from conifer forest (C), shrub(More)
The hydrolysis of urea as a source of ammonia has been proposed as a mechanism for the nitrification of ammonia-oxidizing bacteria (AOB) in acidic soil. The growth of Nitrososphaera viennensis on urea suggests that the ureolysis of ammonia-oxidizing archaea (AOA) might occur in natural environments. In this study, (15)N isotope tracing indicates that(More)
Biological soil disinfestation (BSD) is an effective and environmentally friendly way to suppress soil-borne pathogens. Although it is increasingly used in USA, the Netherlands and Japan, its precise mechanism has not been well quantified so far. Quantitative real-time PCR, denaturing gradient gel electrophoresis and high performance liquid chromatography(More)
INTRODUCTION Nontuberculous mycobacteria (NTM) have emerged as critical opportunistic pathogens of lung diseases recently. Patients with preexisting bronchiectasis are susceptible to NTM. Nevertheless, patients with preexisting bronchiectasis are susceptible to NTM but the prevalence of NTM pulmonary infection in different species and geographical areas is(More)
Although reductive soil disinfestation (RSD) is increasingly used for the control of soil-borne diseases, its impact on the soil microbial community during and after RSD remains poorly understood. MiSeq pyrosequencing, real-time PCR and denaturing gradient gel electrophoresis were performed to investigate the changes of microbial community in the Fusarium(More)
Continuous vegetable cultivation in greenhouses can easily induce soil degradation, which considerably affects the development of sustainable vegetable production. Recently, the reductive soil disinfestation (RSD) is widely used as an alternative to chemical soil disinfestations to improve degraded greenhouse vegetable soils. Considering the importance of(More)