• Publications
  • Influence
Purification and Characterization of an Organic Solvent-Tolerant Lipase from Pseudomonas aeruginosa CS-2
TLDR
An extracellular lipase secreted by Pseudomonas aeruginosa CS-2 was purified to homogeneity about 25.5-fold with an overall yield of 45.5%, indicating the lipase was a metalloenzyme. Expand
Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp. for biodiesel production.
TLDR
It was the first time to use E. coli whole-cell biocatalyst expressing lipase in biodiesel production, and the biodiesel reached a yield of nearly 100% after 12h reaction at the optimal temperature of 15 degrees C, which was the lowest temperature among all the known catalyst in biod diesel production. Expand
Construction of a Novel Shuttle Vector for Use in Gluconobacter oxydans
TLDR
A shuttle vector pZL1 which can replicate both in Gluconobacter oxydans and Escherichia coli was constructed and was used to express a reporter protein wasabi successfully in G. oxydans DSM2003 under the control of the tufB promoter. Expand
Identification of a novel promoter gHp0169 for gene expression in Gluconobacter oxydans.
TLDR
Gluconobacter oxydans can perform rapid incomplete oxidation of many sugars, sugar polyols and alcohols, and this outstanding ability shows a great potential in industrial bioconversion, and a putative promoter sequence, designated as gHp0169, exhibited a stronger promoter activity of NDH-2, indicating its significant value of gene expression in G. oxydan. Expand
Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster
TLDR
The pqqABCDE gene cluster from Gluconobacter oxydans, which is involved in pyrroloquinoline quinone (PQQ) biosynthesis, in Escherichia coli is expressed, resulting in PQQ accumulation in the medium, which suggests that the E. coli tldD gene, which shows high homology to the G. oxydanstld D gene, carries out that function. Expand
Expression of Vitreoscilla Hemoglobin Enhances Cell Growth and Dihydroxyacetone Production in Gluconobacter oxydans
TLDR
Results indicated that the constitutively expressed VHb improved cell growth and DHA production in G. oxydans under different aeration conditions, and oxygen uptake rate was increased in VHB+ strain relative to the control strain during fermentation processes. Expand
Optimization of immobilization for selective oxidation of benzyl alcohol by Gluconobacter oxydans using response surface methodology.
TLDR
This study used the Box-Behnken design and response surface methodology to optimize immobilization of Gluconobacter oxydans in Ca-alginate gel for the production of benzaldehyde in a biphasic system and found that retention activity and oxidative activity of immobilized cells were retained. Expand
Characterization of a Putative Stereoselective Oxidoreductase from Gluconobacter oxydans and Its Application in Producing Ethyl (R)-4-Chloro-3-Hydroxybutanoate Ester
TLDR
A gene encoding an NADH-dependent short-chain dehydrogenase/reductase (gox2036) from Gluconobacter oxydans 621H was cloned and heterogeneously expressed in Escherichia coli and was purified to homogeneity and biochemically characterized. Expand
Co-Expression of an Organic Solvent-Tolerant Lipase and its Cognate Foldase of Pseudomonas aeruginosa CS-2 and the Application of the Immobilized Recombinant Lipase
TLDR
The genes of CS-2 lipase and its cognate foldase were cloned from Pseudomonas aeruginosaCS-2 and showed stability in the presence of some organic solvents and the recombinant lipase was immobilized and used for the synthesis of butyl acetate in heptane. Expand
High cell density fermentation of Gluconobacter oxydans DSM 2003 for glycolic acid production
TLDR
The approach including high-density fermentation of G. oxydans DSM 2003 and bioconversion process was established and proved to be an effective method for glycolic acid production. Expand
...
1
2
3
4
5
...