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Drosha protein levels are translationally regulated during Xenopus oocyte maturation
Drosha is required for the nuclear processing of pri- to pre-miRNAs. Drosha itself is translationally regulated during oocyte-to-egg maturation. Upon completion of meiosis, Drosha mRNA isExpand
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AMPLIFICATION OF PEACH ACC OXIDASE cDNA BY SINGLE SPECIFIC PRIMER AND EXPRESSION IN E.COLI
One specific primer and oligo(dT) 15 were used to amplify about 1.3 kb fragment in samples of cDNA to mRNA isolated from wounded leave of peach ( Prunus persica ). Three different types of PCR clonesExpand
Expression of 6×his-hGM-CSF fusion gene in larvae of silkworm(Bombyx mori) using recombinant baculovirus
Six×his-hGM-CSF fusion gene was inserted in silkworm baculovirus transfer vector pBacPAK8 to construct the recombinant transfer vector pBacPAKHis-GM-CSF.The recombinant transfer vector DNA and linearExpand
Cloning and Sequence Analysis of Human Hepatitis B Virus preS Gene Variants from Hangzhou an d Lanzhou in China
PreS gene fragments were inserted into pBluescript SK after being amplified by PCR from HBV DNA,which was extracted from HBsAg-positive sera(Hangzhou and Lanzhou samples)and the cloned genes wereExpand
Inhibition of BmNPV replication in Bombyx mori cell by dsRNA triggered RNA interference
RNA interference (RNAi) causes degradation of targeted endogenous RNA in many diverse organisms. To investigate the effect of dsRNA on silkworm cells, we transfected three kinds of synthetic dsRNAsExpand
Extraction of mitochondrial DNA from cytoplasmic male sterile and maintainer lines of tuber mustard (Brassica juncea var. tumida Tsen et Lee).
Mitochondrial (mt) DNA has been extracted from paired lines of cytoplasmic male sterile and maintainer lines of tuber mustard based on sucrose shelf. Evaluation of mtDNA by agrose gel electrophoresisExpand
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Analysis of plant mRNA upstream open reading frames
Upstream open reading frames (uORFs) in 5′-untranslated regions (5′-UTRs) of eukaryotic mRNAs play an important role in translation efficiency. Computational analysis of the upstream ATG (uATG) andExpand