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Halophiles, coming stars for industrial biotechnology.
Effects of chromosomal gene copy number and locations on polyhydroxyalkanoate synthesis by Escherichia coli and Halomonas sp.
TLDR
It is demonstrated that chromosome engineering based on active transcriptional site and gene copy number is more feasible for polyhydroxyalkanoate (PHA) synthesis in Halomonas TD08 compared with in E. coli.
CRISPR/Cas9 editing genome of extremophile Halomonas spp.
Cloning of a Novel Arylamidase Gene from Paracoccus sp. Strain FLN-7 That Hydrolyzes Amide Pesticides
TLDR
The results in the present study suggest that AmpA is a good candidate for the study of the mechanism for amide pesticide hydrolysis, genetic engineering of amide herbicide-resistant crops, and bioremediation of amid pesticide-contaminated environments.
Development of an enhanced chromosomal expression system based on porin synthesis operon for halophile Halomonas sp.
TLDR
An inducible expression system was constructed in phaC-deleted Halomonas TD01 by integrating the lac repressor gene into the porin site in the host chromosome, and the stable and strong chromosomal expression method in HalomonAs TD spp.
Polyhydroxyalkanoates, challenges and opportunities.
Promoter Engineering for Enhanced P(3HB- co-4HB) Production by Halomonas bluephagenesis.
TLDR
This study reports an approach to construct a promoter library based on the Pporin core region, namely, from the -35 box to the transcription start site, a spacer and an insulator, suitable for the transcription of many different heterologous genes.
Pilot Scale-up of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Production by Halomonas bluephagenesis via Cell Growth Adapted Optimization Process.
TLDR
A stable and continuous open process for efficient low-cost production of P(3HB-co-4HB) is successfully developed coupling fermentation with the downstream extraction processing.
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