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Rice straw was pretreated using aqueous-ammonia solution at moderate temperatures to enable production of the maximum amount of fermentable sugars from enzymatic hydrolysis. The effects of various operating variables including pretreatment temperature, pretreatment time, the concentration of ammonia and the solid-to-liquid ratio on the degree of lignin(More)
Rice straw was irradiated using an electron beam at currents and then hydrolyzed with cellulase and beta-glucosidase to produce glucose. The pretreatment by electron beam irradiation (EBI) was found to significantly increase the enzyme digestibility of rice straw. Specifically, when rice straw that was pretreated by EBI at 80 kGy at 0.12 mA and 1 MeV was(More)
Phanerochaete chrysosporium is a wood-rot fungus that is capable of degrading lignin via its lignolytic system. In this study, an environmentally friendly fungal pretreatment process that produces less inhibitory substances than conventional methods was developed using P. chrysosporium and then evaluated by various analytical methods. To maximize the(More)
Rice straw was fermented by a wood-rot fungus Dichomitus squalens as a biological pretreatment, to increase the enzymatic digestibility of lignocellulose and promote cellulose hydrolysis. Response surface methodology was employed to optimize the fermentation medium of D. squalens for achieving the maximum volumetric activity of manganese peroxidase. The(More)
Dichomitus squalens, a wood-rotting fungus, can utilize recalcitrant biomass for growth; however, the extracellular metabolic processes involved are not well understood. A systematic target analysis of D. squalens has been carried out using optimized conditions for lignocellulolysis. Seven encoded proteins and 58 metabolites that consistently exhibited(More)
The lignocellulolytic platform of the wood-decaying organism Dichomitus squalens is important for production of biodegradable elements; however, the system has not yet been fully characterized. In this study, using statistical target optimization, we analysed substrate selectivity based on a variety of D. squalens metabolic pathways using combined omics(More)
Plant biomass can be utilized by a lignocellulose-degrading fungus, Phanerochaete chrysosporium, but the metabolic and regulatory mechanisms involved are not well understood. A polyomics-based analysis (metabolomics, proteomics, and transcriptomics) of P. chrysosporium has been carried out using statistically optimized conditions for lignocellulolytic(More)
In order to solve the inefficient problem of long-term biodegradation by wood-decaying fungus, rice straw (RS) was depolymerized using electron beam irradiation-based biodegradation (EBIBB). This environment-friendly program without the use of inhibitory byproducts significantly increased the digestibility and fermentability of RS. Specifically, when(More)
To support the inefficient limitation of long-term biosystem by well-known simultaneous saccharification and fermentation (SSF), electron beam irradiated rice straw (at 80 kGy, 1 MeV, and 0.12 mA) was fermented using fungal-based simultaneous saccharification and fermentation (FBSSF) by saprophytic zygomycetes Mucor indicus. Based on the growth optimization(More)
KSTAR is an advanced tokamak with fully superconducting coils for the steady state research, which began in December 1995 and was completed in July 2008. As a single science project, it has been marked as the largest construction project in the history of Korea. During the construction period, we encountered several challenges, such as technical issues,(More)