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Legumes play a crucial role in nitrogen supply to grass-legume mixtures for ruminant fodder. To quantify N transfer from legumes to neighbouring plants in multi-species grasslands we established a grass-legume-herb mixture on a loamy-sandy site in Denmark. White clover (Trifolium repens L.), red clover (Trifolium pratense L.) and lucerne (Medicago sativa(More)
We carried out field experiments to investigate if an agricultural grassland mixture comprising shallow- (perennial ryegrass: Lolium perenne L.; white clover: Trifolium repens L.) and deep- (chicory: Cichorium intybus L.; Lucerne: Medicago sativa L.) rooting grassland species has greater herbage yields than a shallow-rooting two-species mixture and pure(More)
This study was conducted to answer the question of whether clover can absorb asparagine in the presence and absence of inorganic nitrogen, as well as to determine the resulting concentration of post-uptake compounds closely involved in asparagine metabolism. Clover was grown at two asparagine concentrations (10 μM and 1 mM) supplied in both the absence and(More)
Application of carbon (C) and nitrogen (N) isotopes is an essential tool to study C and N flows in plant-soil-microorganisms systems. When targeting single plants in a community the tracers need to be added via e.g., leaf-labeling or stem-feeding approaches. In this study we: (i) investigated if bicarbonate can be used to introduce 14C (or 13C) into white(More)
While N2-fixation in diversified grasslands including forage legumes and non-legumes has been widely studied, N2-fixation in swards containing only forage legumes remains unclear. In this study, we investigated N2-fixation in pure stands and mixtures of three forage legumes. N2-fixation, dry matter (DM) and nitrogen (N) yields were quantified in a field(More)
There is substantial evidence that legume-derived Nitrogen (N) is transferred to neighboring non-legumes in grassland mixtures. However, there is sparse information about how deep rooted non-legume forage herbs (forbs) influence N transfer in multi-species grasslands. Red clover (Trifolium pretense L.) was grown together with perennial ryegrass (Lolium(More)
Estimating the potential for direct plant acquisition of organic N, in particular amino acids, requires assessment of their turnover times in soil. It is well known from 14C studies that mineralization of amino acids occurs within hours, but mineralization to 14CO2 does not indicate the rate of disappearance of the intact amino acid or the possible(More)
Leaching of dissolved inorganic N (DIN) and dissolved organic N (DON) is a considerable loss pathway in grassland soils. We investigated the white clover (Trifolium repens) contribution to N transport and temporal N dynamics in soil solution under a pure stand of white clover and white clover-ryegrass (Lolium perenne) mixed stand. The temporal white clover(More)
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