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A PCR-based ‘molecular tool box’, based on a region of the ras-related protein gene Ypt 1, was developed for the identification of 15 Phytophthora species that damage forests and trees: P. cactorum , P. cambivora , P. cinnamomi , P. citricola , P. europaea , P. inundata , P. lateralis , P. megasperma , P. nemorosa , P. kernoviae , P. pseudosyringae , P.(More)
Phytophthora fragariae Hickman, which causes strawberry red stele and raspberry root rot, is a quarantine organism for which specific and sensitive detection methods are required to test the health of planting material. Sequences of the internal transcribed spacer regions of the ribosomal gene repeat (rDNA) were used to develop primers for P. fragariae in a(More)
Phytophthora fragariae, the cause of strawberry red stele disease, is a quarantine pathogen in Europe. Detecting low levels of infection requires sensitive and specific methods. In the past, Dutch and English inspection services have used bait plants to test strawberry propagation stocks destined for export. Increasingly though, PCR is being incorporated(More)
Six primers based on the sequence of the flanking and coding regions of the elicitin gene ParA1 of Phytophthora nicotianae were tested for specific detection of the fungus by the polymerase chain reaction (PCR). One combination, IL7/IL8, with IL7 in a flanking region and IL8 in a coding region of the gene, gave an intense 378 bp signal with a diverse(More)
A PCR-based method was developed for the identification and detection of Phytophthora capsici in pepper plants. Three PCR primers (CAPFW, CAPRV1 and CAPRV2) specific for P. capsiciwere designed based on the sequence of its internal transcribed spacer regions. CAPFW/CAPRV1 amplify a 452 bp product from P. capsici DNA whereas CAPFW/CAPRV2 a 595 bp fragment;(More)
A retrospective review of a limited number of adolescents with implants was conducted to compare the behavior of these implants with studies in which implants had been placed in growing animals. Growth of the facial skeleton is also reviewed. Implants placed in the growing alveolus behave like ankylosed teeth and become submerged as the surrounding bone(More)
Brigham and Women’s Hospital, 75 Francis St., Boston, Massachusetts 02115 Georgetown University, 2115 Wisconsin Avenue NW, Suite 603 Washington, DC 20057 Kitware Inc, 28 Corporate Drive, Clifton Park, New York 12065 Johns Hopkins University, B26 New Engineering Bldg, 3400 North Charles St, Baltimore, MD 21218 CIMIT, 65 Landsdowne Street, Suite 200,(More)