Jim Costello

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A simple method is described for the measurement of urinary oxalate. Oxalate decarboxylase is coupled with NAD+ requiring formate dehydrogenase and the result recorded spectrophotometrically. Accurate determination can be carried out either on urine or a citrate extract or urine. Using the citrate extract procedure, the urinary samples can be stored for at(More)
The methods used for determination of oxalate in blood are reviewed, and the advantages and disadvantages of the two basic approaches--direct methods and in vivo isotope-dilution techniques--are compared. Possible reasons for the previous discrepancies between direct and isotopic methods are discussed, as are the effects of protein binding, sample handling,(More)
The goal of this 'Birds of a Feather' session will be to share our current knowledge about XML for web site content management in government settings and explore some areas for future research. Specifically, the session will address topics such as the benefits and challenges faced by public managers when attempting to use XML for managing their web sites.(More)
We describe a sensitive simplified enzymic method for serum oxalate determination. After serum ultrafiltration, precipitation, and extraction, oxalate is decarboxylated and the resultant formate is measured spectrophotometrically by using NAD-requiring formate dehydrogenase. There have been several recent methods for determining serum oxalate in man (1-8),(More)
Chemiluminescent acridinium ester labels are widely used in clinical diagnostics especially in automated immunochemistry analyzers such as Siemens Healthcare Diagnostics' ADVIA Centaur systems. Although, chemiluminescence from acridinium compounds was discovered more than 50 years ago, details regarding the excitation process are still not well understood(More)
The effects of altered acid-base balance on the production of urea and the metabolism of glutamine were investigated in the isolated perfused liver and hindquarter of the rat. In the isolated perfused rat liver, lowering of perfusate pH without altering bicarbonate concentration significantly reduced urea production and increased net glutamine synthesis,(More)
Six siblings with Bartter's syndrome were studied. Increased urinary immunoreactive prostaglandin E (iPgE) was corrected by administration of the prostaglandin synthetase inhibitors, indomethacin, ibuprofen and meclofenamate. In addition, plasma potassium rose, plasma renin activity and angiotensin resistance decreased, and the exaggerated natriuresis(More)
Acknowledgments T his report is based on the lessons learned from CTG's XML Testbed. The success of the Testbed rested on the enthusiastic participation of five New York State (NYS) agencies who committed to extensive hours of workshops, training, and prototype development. CTG extends its thanks to the Several members of the CTG staff made many important(More)
Daily ingestion of 8 g of pure ascorbic acid by 8 normal subjects for 7 days did not, in contrast to previous reports in the literature, significantly alter urinary or plasma oxalate during or after ingestion. When urine with raised ascorbate values was heated at 100 degrees C for 30 min, a significant increase in urinary oxalate concentration was observed.(More)