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A continuous flow method is described as applied to the investigation of microbiological processes taking place in soil samples. The principle of the method and its technical arrangement are also presented in detail. The applicability of the continuous flow method to microbiological research is discussed. Описан непрерывный проточный метод, применяемый, для(More)
The oxidation ofp-hydroxybenzoic acid, quinic acid, vanillin and coumarin in soil was studied. With vanillin, and particularly with coumarin, the lag phase for oxygen consumption was longer and the rate of oxygen consumption attained more than one peak. In soil preincubated with the relevant substrate, the second dose of the same substrate was oxidized more(More)
Decomposition of preparations of various fractions of fulvio acids in pure cultures of bacteria and in the soil was investigated. In the soils enriched with fulvic acids the amount of bacteria increased, oxygen consumption and formation of carbon dioxide followed a typical sigmoid curve. The above measurements indicated that mineralization occurred after a(More)
The protease activity of sterile roots of wheat was zero or very low, so that the determined values did not exceed limits of the experimental error. Roots colonized by microorganisms had a significant protease activity. The activity of protease on seeds and roots of the plants growing in a medium inoculated with the soil microflora was higher than in cases(More)
A soil not naturally containing montmorillonite (M) was amended with approximately 5, 10 or 20% M or kaolinite (K), maintained in a greenhouse under periodic cultivating and alternate wetting and drying for more than two years, and then used in perfusion studies. The incorporation of M enhanced the rate of both heterotrophic degradation of glycine and(More)
Continuous flow technique was used to study the formation of organic acids and of carbon dioxide during anaerobic breakdown of glucose in soil. Carbon dioxide, formic, acetic, butyric and lactic acids were the main products of anaerobic decomposition of glucose. However, succinic acid, α-ketoglutaric acid and fumarie or glutarie acids could be detected also(More)
The effect of glucose on the decomposition of plant material in soil was studied. Soil samples were enriched with different fractions of labelled alfalfa: the soluble dialyzable fraction, the soluble nondialyzable fraction, cellulose-lignin, lucerne meal. The added substances were decomposed for 42 days. One soil sample in every experimental variant was(More)