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BACKGROUND Next-generation-sequencing (NGS) technologies combined with a classic DNA barcoding approach have enabled fast and credible measurement for biodiversity of mixed environmental samples. However, the PCR amplification involved in nearly all existing NGS protocols inevitably introduces taxonomic biases. In the present study, we developed new(More)
This work reports a newly developed two-step hydrothermal method for the synthesis of monodispersed colloidal carbon spheres (CCS) under mild conditions. Using this approach, monodispersed CCS with diameters ranging from 160 to 400 nm were synthesized with a standard deviation around 8%. The monomer concentration ranging from 0.1 to 0.4 M is in favor of(More)
—Thresholding is an important topic for image processing, pattern recognition and computer vision. Selecting thresholds is a critical issue for many applications. The fuzzy set theory has been successfully applied to many areas, such as control, image processing, pattern recognition, computer vision, medicine, social science, etc. It is generally believed(More)
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