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OBJECTIVE To investigate the effects of red orpiment on cell morphology, expression of promyelocytic leukemia (PML) mRNA and its protein localization in NB4 and HL-60 cell lines. METHODS Cell morphology was assayed by Wright's staining and fluorescence staining, while PML mRNA expression was determined by RT-PCR. PML protein localization by evaluated by(More)
OBJECTIVE To investigate PML gene and protein expression and localization in leukemia cell lines. METHODS Cell morphology was assayed by Wright and fluorescence stain, PML mRNA expression by RT-PCR, and PML protein localization by immunofluorescence. RESULTS (1) Differentiation was observed by morphology in NB4 and HL-60 cells after treatment with(More)
OBJECTIVE To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray. METHODS Two cDNA probes were made from mRNA of untreated or arsenic sulfide treated NB4 cells. The cells were labelled with Cy3 or Cy5 fluorescence dyes individually, hybridized with cDNA microarray, and scanned for fluorescent(More)
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