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The ultrastructure of the capillaries and their permeability to lanthanum ions and ferritin in the corpus callosum was examined in postnatal rat brain. In 1 and 7-d-old rats, numerous pinocytotic vesicles were observed in the endothelial cytoplasm of the callosal capillaries. Tight junctions were present between adjacent endothelial cells which were(More)
Interferon-gamma when given intraperitoneally by single daily injection into one-day-old rats upregulated the expression of major histocompatibility complex class I antigen on ramified microglial cells in cerebral cortex and induced the expression of major histocompatibility complex class II antigen both on amoeboid and ramified microglial cells present in(More)
The effects of bacterial lipopolysaccharide (LPS) on the expression of surface antigens including major histocompatibility complex (MHC) and complement type 3 (CR3) receptors on microglial cells in the corpus callosum in postnatal rat brain were investigated. When LPS was injected intravenously (i.v.) in 1-d-old rats, the immunostaining of callosal amoeboid(More)
In primary cultures of cerebellar granule cells, the gamma aminobutyric acid B (GABAB) receptor couples to an inhibitory mechanism of adenylate cyclase. The inhibition of adenylate cyclase can be observed either by the measurement of cellular cyclic AMP content or by in vitro measurement of adenylate cyclase from plasma membrane of these cerebellar granule(More)
The induction of major histocompatibility complex (MHC) class II antigen on amoeboid microglia cells (AMC) by lipopolysaccharide (LPS) and interferon-gamma (INF-gamma) in early postnatal rat brain was studied by immunohistochemistry. In newborn rats given successive intraperitoneal injections of LPS or INF-gamma and killed at the age of 7 days, MHC class II(More)
Cultured granule cells prepared from 8-day postnatal rats were used for the study of carbachol-induced phosphoinositide turnover. The addition of carbachol induced a 20- to 30-fold increase in [3H]inositol monophosphate (IP1) accumulation within 30 min in the presence of 20 mM LiCl in granule cells prelabeled with [3H]myoinositol. Carbachol also stimulated(More)
Amoeboid microglial cells (AMC) in the corpus callosum were selectively labelled following a single intraperitoneal (i.p.) injection of the fluorescent dye, rhodamine isothiocyanate (RhIc) into postnatal rats. The frequency of RhIc-labelled cells varied with age, with the largest number occurring in 7-d-old animals. Thereafter, the labelled cells declined(More)
Phosphatidylinositol (Ptdlns) turnover has been studied in the primary culture of granule cells dissociated from the cerebellum of postnatal rat. Addition of serotonin (5-hydroxytryptamine, 5-HT) caused an increase (300-600% of control) of [3H]inositol monophosphate (IP1) accumulation in the presence of LiCl in cells prelabeled with [3H]myo-inositol. The(More)
To investigate the capability of Sprague-Dawley rat bone marrow stromal cells to secrete glial cell line-derived neurotrophic factors (GDNF), we detected expression of GDNF messenger RNA and protein in bone marrow stromal cells of Sprague-Dawley rats by reverse-transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA),(More)
When HRP was injected intraperitoneally (i. p.), labelled amoeboid microglial cells (AMC) were consistently localized in the subcortical white matter and circumventricular zones in early postnatal (1 and 7 days old) but absent in late postnatal (14-day-old) rats. The ingested HRP disappeared from the labelled cells 5 days after IP injection. Subcutaneous(More)