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We present a method to separate clustered nuclei from fluorescence microscopy cellular images, using shape markers and marking function in a watershed-like algorithm. Shape markers are extracted using an adaptive H-minima transform. A marking function based on the outer distance transform is introduced to accurately separate clustered nuclei. With synthetic(More)
Snakes, or active contour models, have been widely used in image segmentation. However, most present snake models do not discern between positive and negative step edges. In this paper, a new type of dynamic external force for snakes named dynamic directional gradient vector flow (DDGVF) is proposed that uses this information for better performance. It(More)
In this paper, an automated method of boundary detection of the left ventricle (LV) is proposed. The method uses a watershed transform and morphological operation to locate the region containing the LV, then performs snake deformation with a multiscale directional edge map for the detection of the endocardial boundary of the LV
Lung injury caused by influenza virus infection is widespread. Understanding lung damage and repair progression post infection requires quantitative spatiotemporal information on various cell types mapping into the tissue structure. Based on high content images acquired from an automatic slide scanner, we have developed algorithms to quantify cell(More)
Automatic detection of region of interest and center point of left ventricle using watershed segmentation. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must(More)
Graphene was recently shown to support deep subwavelength surface plasmons at terahertz frequencies characterized by low energy loss and strong field localization, both highly desirable. The properties of graphene can be locally tuned by applying an external gate voltage or by the adsorption of organic molecules that lead to doping through charge transfer.(More)
Image segmentation is to separate the image domain into sub-domains according to some optimization rule. Variational level-set image segmentation based on the Mumford-Shah model, together with level set formulation, leads to complicate implementation and expensive computational time. To overcome the difficulty in solving the complex coupled level set(More)
Image segmentation is to separate the image domain into local regions. Implicit active contour models via the level-set method can provide smooth and closed contours. The major category is based on the Mumford-Shah image component decomposition model. Approximating the image domain by a set of homogeneous regions, segmentation based on the piecewise(More)