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We present a method to separate clustered nuclei from fluorescence microscopy cellular images, using shape markers and marking function in a watershed-like algorithm. Shape markers are extracted using an adaptive H-minima transform. A marking function based on the outer distance transform is introduced to accurately separate clustered nuclei. With synthetic(More)
Snakes, or active contour models, have been widely used in image segmentation. However, most present snake models do not discern between positive and negative step edges. In this paper, a new type of dynamic external force for snakes named dynamic directional gradient vector flow (DDGVF) is proposed that uses this information for better performance. It(More)
In this paper, an automated method of boundary detection of the left ventricle (LV) is proposed. The method uses a watershed transform and morphological operation to locate the region containing the LV, then performs snake deformation with a multiscale directional edge map for the detection of the endocardial boundary of the LV.
Automatic detection of region of interest and center point of left ventricle using watershed segmentation. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must(More)
Graphene was recently shown to support deep subwavelength surface plasmons at terahertz frequencies characterized by low energy loss and strong field localization, both highly desirable. The properties of graphene can be locally tuned by applying an external gate voltage or by the adsorption of organic molecules that lead to doping through charge transfer.(More)