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Spt6 enhances the elongation rate of RNA polymerase II in vivo
RNAi depletion of Spt6 reveals its broad requirement during different stages of development, reducing the elongation rate from ∼1100 to 500 bp/min. Expand
Dynamics of heat shock factor association with native gene loci in living cells
The results provide strong evidence that activated HSF is stably bound to DNA in vivo and that turnover or disassembly of transcription activator is not required for rounds of hsp70 transcription, and indicate that transcription activators display diverse dynamic behaviours in their associations with targeted loci in living cells. Expand
Intranuclear distribution and local dynamics of RNA polymerase II during transcription activation.
Evaluating the location, movement, and transcriptional dynamics of Drosophila heat shock (HS) genes both by two-photon microscopy in live polytene nuclei and by FISH in diploid nuclei reveals dynamics resembling combinations of these Hsp70 transcription modes. Expand
P-TEFb Is Critical for the Maturation of RNA Polymerase II into Productive Elongation In Vivo
- Zhuoyu Ni, Abbie Saunders, +4 authors J. Lis
- Medicine, Biology
- Molecular and Cellular Biology
- 10 December 2007
It is demonstrated that P-TEFb is critical for the transition of Pol II into a mature transcription elongation complex in vivo and in the continued presence of P- TEFb inhibitor, Pol II levels across the gene eventually recovered. Expand
Blinking and nonradiant dark fraction of water-soluble quantum dots in aqueous solution.
- Jie Yao, D. Larson, Harshad D. Vishwasrao, W. Zipfel, W. Webb
- Chemistry, Medicine
- Proceedings of the National Academy of Sciences…
- 4 October 2005
Water-soluble quantum dots are characterizes photophysical pathologies of qdots in biologically relevant environments rather than adhered on dielectric surfaces and methods that are useful for studying various bioapplicable nanoparticles are described. Expand
The zipcode-binding protein ZBP1 influences the subcellular location of the Ro 60-kDa autoantigen and the noncoding Y3 RNA.
It is reported that the zipcode-binding protein ZBP1 influences the subcellular localization of both Ro and the Y3 RNA, and data indicate that Z BP1 may function as an adapter to export the Ro/Y3 RNA complex from nuclei. Expand
Imaging transcription in living cells.
The current state of the field and the progress necessary to achieve a more complete understanding of the various steps in transcription are reported on. Expand
Regulation of Poly(ADP-ribose) Polymerase-1-dependent Gene Expression through Promoter-directed Recruitment of a Nuclear NAD+ Synthase*
- Tong Zhang, Jhoanna G Berrocal, +6 authors W. Kraus
- Biology, Medicine
- The Journal of Biological Chemistry
- 13 February 2012
Using a variety of biochemical, molecular, cell-based, and genomic assays, this work shows that NMNAT-1 and PARP-1 physically and functionally interact at target gene promoters in MCF-7 cells, revealing a new mechanism for the regulation of gene expression by NAD+. Expand
Spatial compartmentalization at the nuclear periphery characterized by genome-wide mapping
This work has shown that genomic regions associated with the NL are characterized by the paucity of active histone modifications in mammalian cells, and has revealed novel connections between subnuclear gene positioning, hist one modifications and gene expression. Expand
Role of pH in structural changes for Pin1 protein: an insight from molecular dynamics study
- Yu Wang, L. Xi, Jie Yao, Jiao Yang, Lin-Fang Du
- Chemistry, Medicine
- Journal of Molecular Modeling
- 17 July 2014
The present study shows that one protein domain (PPIase domain) is more sensitive than the other one (WW domain) in Pin1 protein, and also shows that the integrality of the two conserve tryptophan in one domain (WW) is important in response to low pH. Expand