• Publications
  • Influence
l-Fucose utilization provides Campylobacter jejuni with a competitive advantage
This work identifies a previously unrecorded metabolic pathway in select strains of C. jejuni associated with a virulent lifestyle that is deficient in fucose uptake and demonstrate a competitive disadvantage when colonizing the piglet model of human disease, which is not paralleled in the colonization of poultry. Expand
Structural Characterization of Campylobacter jejuni Lipooligosaccharide Outer Cores Associated with Guillain-Barré and Miller Fisher Syndromes
It is concluded that molecular mimicry between gangliosides and C. jejuni LOS is the presumable pathogenic mechanism in most cases of C.Jejuni-related Guillain-Barré syndrome, however, the findings suggest that in some cases, other mechanisms may play a role. Expand
The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168.
The structural information presented here will aid in the identification and characterization of specific enzymes that are involved in the biosynthesis of these structures and may lead to the discovery of potential therapeutic targets. Expand
The crucial role of Campylobacter jejuni genes in anti-ganglioside antibody induction in Guillain-Barre syndrome.
It is demonstrated, for the first time, to the knowledge, that specific bacterial genes are crucial for the induction of anti-ganglioside antibodies. Expand
Phosphorylation of the Lipid A Region of Meningococcal Lipopolysaccharide: Identification of a Family of Transferases That Add Phosphoethanolamine to Lipopolysaccharide
ABSTRACT A gene, NMB1638, with homology to the recently characterized gene encoding a phosphoethanolamine transferase, lpt-3, has been identified from the Neisseria meningitidis genome sequence andExpand
Host-derived sialic acid is incorporated into Haemophilus influenzae lipopolysaccharide and is a major virulence factor in experimental otitis media
It is shown that in capsule-deficient (nontypeable) strains, sialic acid, a terminal residue of the core sugars of H. influenzae lipopolysaccharide (LPS), is a critical virulence factor in the pathogenesis of experimental otitis media in chinchillas and play a key role in pathogenicity of nontypeable H. Influenzae disease. Expand
Analysis of Campylobacter jejuni capsular loci reveals multiple mechanisms for the generation of structural diversity and the ability to form complex heptoses
For the first time, the commonality and function of a second heptose biosynthetic pathway for Campylobacter CPS independent of the pathway for lipooligosaccharide (LOS) biosynthesis is demonstrated and a novel heptosyltransferase utilized by this alternate pathway is identified. Expand
Detection of Conserved N-Linked Glycans and Phase-variable Lipooligosaccharides and Capsules from Campylobacter Cells by Mass Spectrometry and High Resolution Magic Angle Spinning NMR Spectroscopy*
The presence of this common heptasaccharide in multiple campylobacter isolates demonstrates the conservation of the N-linked protein glycosylation pathway in this organism and describes the first report of HR-MAS NMR detection of N- linked glycans on glycoproteins from intact bacterial cells. Expand
Effects of Sequential Campylobacter jejuni 81-176 Lipooligosaccharide Core Truncations on Biofilm Formation, Stress Survival, and Pathogenesis
Novel roles for the C. jejuni LOS are uncovered, a mouse competition model is provided, insight is provided into the contribution of specific LOS core moieties to stress survival and pathogenesis, and novel resistance to complement-mediated killing is highlighted. Expand
High-level antibiotic resistance in Pseudomonas aeruginosa biofilm: the ndvB gene is involved in the production of highly glycerol-phosphorylated beta-(1->3)-glucans, which bind aminoglycosides.
It is revealed that the highly glycerol-phosphorylated beta-(1-->3)-glucans are lacking in the ndvB mutant, and it is shown that these glucans are capable of direct binding with the aminoglycoside antibiotic kanamycin, filling a gap in the understanding of the relationship between biofilm, cyclic glucans and high-level antibiotic resistance. Expand