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Intelligence optimization algorithms are a large class of probabilistic optimization algorithms, which solve varieties of complex optimization problems by mimicking the physical or biological phenomena, including Simulated Annealing, Genetic Algorithms, Ant Colony Optimization, and Particle Swarm Optimization and so on. These algorithms are successful in(More)
A dual route for anaerobic succinate production was engineered into Corynebacterium glutamicum. The glyoxylate pathway was reconstructed by overexpressing isocitrate lyase, malate synthase and citrate synthase. The engineered strain produced succinate with a yield of 1.34 mol (mol glucose)−1. Further overexpression of succinate exporter, SucE, increased(More)
2-Deoxy-d-ribose 5-phosphate aldolase (DERA) accepts a wide variety of aldehydes and is used in de novo synthesis of 2-deoxysugars, which have important applications in drug manufacturing. However, DERA has low preference for non-phosphorylated substrates. In this study, DERA from Klebsiella pneumoniae (KDERA) was mutated to increase its enzyme activity and(More)