Jian-zhi Gao

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AIM To investigate the prognostic and clinicopathological significance of glypican-3 (GPC3) overexpression in hepatocellular carcinoma (HCC). METHODS Publications were searched using PubMed, EMBASE, the Cochrane Library and the Chinese Biomedical Literature Database up to March 2013. Inclusion and exclusion criteria were established to screen eligible(More)
Cell division cycle 20 (CDC20) encodes a regulatory protein interacting with the anaphase-promoting complex/cyclosome (APC/C) in the cell cycle and plays important roles in tumorigenesis and progression of multiple tumors. The present study aimed to investigate the clinical significance of CDC20 in hepatocellular carcinoma (HCC) and the role of CDC20 in the(More)
The aim of the present study was to explore the effects of curcumin in combination with bevacizumab on the vascular endothelial growth factor (VEGF)/VEGF receptor (VEGFR)/K-ras pathway in hepatocellular carcinoma. A total of 30 Sprague Dawley (SD) rats were randomly divided into five groups: Control, model, curcumin, VEGF blocker, and curcumin + VEGF(More)
  • J Gao, Q Tao, D Ma
  • Zhonghua shi yan he lin chuang bing du xue za zhi…
  • 2001
OBJECTIVE To express the HCV E1 gene in E. coli cells and to demonstrate its clinical significance in detection of anti-HCV E1 antibodies. METHODS The expression vector was constructed by ligation of HCV E1 sequence, which was amplified by RT-PCR methods from 50 microliters of HCV RNA positive serum using primers specific to the HCV E1 sequence, to the(More)
Hyperlipidemia may lead to endothelial injury, due to its effects on homocysteine and vascular endothelial growth factor in the serum, and the mRNA expression levels of peroxisome proliferator-activated receptor-γ (PPARγ), and caspase-3 and -8 in the vascular wall. In order to prevent and mitigate the high-fat state that results from endothelial injury,(More)
OBJECTIVE To prepare hybridoma cell lines which secrete anti-HCV recombinant NS5a protein monoclonal antibodies, and to evaluate their usage in investigating the distribution of HCV NS5a antigen in liver tissue. METHODS The hybridoma cell lines were raised using the conventional protocols. The immunohistochemistry of paraffin embedded liver tissue was(More)
AIM To prepare hybridoma cell lines that secrete monoclonal antibodies against hepatitis C virus (HCV) recombinant proteins NS3 and NS5 and to evaluate their use in the study of HCV NS3 and NS5 antigen distribution in human liver tissue. METHODS Hybridoma cell lines were generated using spleen cells from BALB/C mice immunized with recombinant NS3 and NS5(More)
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