AIM
To express Fd fragment and L chain of human anti-keratin Fab in E.coli BL21(DE3) respectively and obtain human anti-keratin Fab by renaturation in-vitro.
METHODS
Genes of L chain and Fd… (More)
AIM
To construct an eukaryotic expression vector containing the gene of anti-keratin antibody and express it in CHO(dhfr(-)) cells.
METHODS
Both the V(L) and V(H) genes of an anti-keratin Fab from… (More)
To construct and express Hsp70-HSV2gD fusion protein. Genes of Hsp70 and HSV-2gD were subcloned into vectors pGEX-4T-1 respectively. After confirmed by DNA sequence analysis, the recombinant plasmids… (More)
AIM
To express human anti-keratin Fab in Pichia pastoris secretively and optimize the expression condition.
METHODS
Genes of kappa chain and Fd fragment of anti-keratin antibody from the plasmids… (More)