Jian Ping Liu

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In this morphometric analysis of immunoreactive serotonin (5-HT) and tyrosine hydroxylase (TH) neurons in culture, 5-HT and the MAO inhibitor nialamide influenced the survival, cell body size and neurite outgrowth of embryonic day 14 (E14) 5-HT neurons after treatment from 1-3 days in vitro (DIV), but did not significantly affect E14 or E15 TH neurons of(More)
GABA exerts a variety of trophic influences on developing brain cells, as reviewed in this issue. During early stages of brain development, GABAergic axons course through regions where other neurotransmitter phenotypes are being generated. This raises the question of whether GABA may influence the ontogeny of these neurotransmitter systems in the embryonic(More)
The inhibitory neurotransmitter GABA may act as a trophic signal for developing monoamine neurons in embryonic rat brain, because GABA neurons and their receptors appear in brainstem during generation of monoamine neurons. To test this hypothesis, we used dissociated cell cultures from embryonic day 14 rat brainstem, which contains developing serotonin(More)
In embryonic rat brain, serotonin (5-HT) acts as a differentiation signal for 5-HT neurons and their target cells during midgestation. Serotonin receptors expressed during this period include the 5-HT(1A) subtype, which may mediate some of these developmental effects. Using the highly sensitive method of competitive RT-PCR, we quantified the effects of(More)
To study the phenotypic specificity of S-100 beta and insulin-like growth factor II (IGF-II) for developing monoamine neurons, serotonin (5-HT) neurons from the embryonic day 14 (E14) rostral raphe or dopamine (TH) neurons from the substantia nigra/ventral tegmental area were cultured for 3 days in vitro (3 DIV) in the presence of these factors.(More)
We have previously shown that GABA acts as a trophic signal for monoamine neurons in embryonic day 14 (E14) rat brainstem cultures [Liu et al., J Neurosci 1997a; 17:2420-2428]. The organochlorine pesticide dieldrin and the classical GABA(A) receptor antagonist bicuculline interfere with the trophic actions of GABA and alter expression of several GABA(A)(More)
The expression of mRNAs for two 5-HT receptors (5-HT1C, 5-HT2) has been investigated by evaluating in situ hybridization in the prenatal rat CNS. At Embryonic Day 14 (E14), the highest signal for 5-HT1C was found in the choroid plexus, while the marginal/intermediate (m/i) zones of the midbrain, brain stem (including monoaminergic groups), and spinal cord(More)
To test the hypothesis that glia mediate interactions between embryonic serotonergic (5-HT) neurons and dopamine neurons, we studied the effects of 5-HT in co-cultures of E14 raphe neurons of mesencephalic dopamine neurons and radial glia/astrocytes derived from the same (homotypic) or opposite (heterotypic) brain region using a dose (10(-5) M) that would(More)
Cyclodiene organochlorine pesticides, such as dieldrin, inhibit gamma-aminobutyric acid (GABA)ergic neurotransmission by blocking the Cl- channel of GABA(A) receptors. This action may make the developing nervous system especially vulnerable to these neurotoxins, which could interfere with the trophic actions of GABA on developing neurons and alter(More)
Evidence from the developing serotonergic system provides support for the working hypothesis that an important function of receptors expressed by developing glia may be to mediate the effects of neurotransmitters on growth and differentiation of the neurons that utilize them, by regulating the production of glial-derived neurotrophic factors. Regional(More)