Jian-Ping An

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The basic helix-loop-helix (bHLH) Leu zipper transcription factor MYC2 is an important regulator in the Jasmonic acid (JA) signaling pathway. In this study, the apple MdMYC2 gene was isolated and cloned on the basis of its homology with Arabidopsis thaliana MYC2. Quantitative real time PCR (qRT-PCR) analysis demonstrated that MdMYC2 transcripts were induced(More)
Glucose induces anthocyanin accumulation in many plant species; however, the molecular mechanism involved in this process remains largely unknown. Here, we found that apple hexokinase MdHXK1, a glucose sensor, was involved in sensing exogenous glucose and regulating anthocyanin biosynthesis. In vitro and in vivo assays suggested that MdHXK1 interacted(More)
The plant specific lateral organ boundaries domain (LBD) family of transcription factors (TFs) is involved in many aspects of plant growth and development. In this study, MdLBD13, a nitrate-induced LBD family gene from the apple (Malus × domestica), was isolated and characterized. Overexpression of MdLBD13 repressed anthocyanin biosynthesis by reducing(More)
MAX2 (MORE AXILLARY GROWTH2) is involved in diverse physiological processes, including photomorphogenesis, the abiotic stress response, as well as karrikin and strigolactone signaling-mediated shoot branching. In this study, MdMAX2, an F-box protein that is a homolog of Arabidopsis MAX2, was identified and characterized. Overexpression of MdMAX2 in apple(More)
The basic leucine zipper (bZIP) transcription factor HY5 plays a multifaceted role in plant growth and development. Here the apple MdHY5 gene was cloned based on its homology with Arabidopsis HY5. Expression analysis demonstrated that MdHY5 transcription was induced by light and abscisic acid treatments. Electrophoretic mobility shift assays and transient(More)
Jasmonate (JA) and ethylene are involved in the regulation of the aluminum (Al)-induced growth inhibition. Although it has been reported that JA enhances Al-induced root-growth inhibition, its role in the regulation of growth interplaying with ethylene is still not well understood. In this study, we investigated the mechanism underlying the effect of apple(More)
Cold stress is a major external stimulator that affects crop quality and productivity. The CBF cold regulatory pathway has been regarded as a master regulator in the response to cold stress. In this study, we found that the apple bZIP transcription factor, MdHY5, was responsive to cold treatment both at the transcriptional and at the post-translational(More)
RING-finger-containing E3 ubiquitin ligases play important roles in plant response to biotic and abiotoc stresses. In this study, through homology analysis, a Malus× domestica MYB30-Interacting E3 Ligase 1 gene, MdMIEL1, was identified and subsequently cloned from apple ‘Gala’ (Malus×domestica). MdMIEL1 contained a zinc finger domain close to N-terminus and(More)
MdMYB1 is an important regulator for anthocyanin accumulation in apple (Malus × domestica). Here, an apple RING E3 ligase MdMIEL1 was screened out as a partner of MdMYB1 with a yeast two-hybrid approach. Pull-down, BiFC and coimmunoprecipitation assays further verified the interaction between MdMIEL1 and MdMYB1 proteins. Subsequently, in vitro and in vivo(More)
Cytochrome P450s play an important role in plant growth and are involved in multiple stresses response. However, little is known about the functions of cytochrome P450s in apple. Here, a Malus × domestica cytochrome P450 monooxygenase 1 gene, MdCYPM1, was identified and subsequently cloned from apple 'Gala' (Malus × domestica). To verify the functions of(More)
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