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Lung cancer is the leading cause of cancer deaths. Non-small-cell lung cancer (NSCLC) is the major type of lung cancer. The aim of this study was to characterize the expression profiles of miRNAs in adenocarcinoma (AC), one major subtype of NSCLC. In this study, the miRNAs were detected in normal, adjacent, and tumor tissues by next-generation sequencing.(More)
It is reported that different microRNA (miRNA) profiles can be detected in the blood of cancer patients. We investigated that whether the key serum miRNAs could discriminate patients with and without breast cancer. This study was divided into three parts: (1) miRNA marker discovery using SOLiD sequencing-based miRNA profiling on cancerous and adjacent(More)
BACKGROUND Circulating miRNAs, as a new family of miRNAs existing in plasma and serum, had shown great potential to serve as a novel biomarker in body fluid for non-invasive diagnosis and prognosis of plenty kinds of disease, such as cancer and prenatal screening. METHODS In this present study, we analyzed the expression profiles of circulating miRNAs in(More)
BACKGROUND To gain insight into potential roles of isomiR spectrum and isomiRs with 3' additions in pre-eclampsia, we performed a comprehensive survey of miRNA repertoire and 3' addition events from placental samples with different degrees of pre-eclampsia by applying SOLiD sequencing platform. PRINCIPAL FINDINGS Over 30% isomiRs were detected with 3'(More)
Exosomes are small membrane-bound vesicles secreted by most cell types. Exosomes contain various functional proteins, mRNAs and microRNAs (miRNAs) that could be used for diagnostic and therapeutic purposes. How we should store the samples before RNA isolation and whether those long term stored samples could be used for circulating RNA investigation because(More)
The small non-coding important regulatory molecules, microRNAs (miRNAs), have been widely and deeply studied especially combining high-throughput sequencing technologies. Here, we attempted to track detailed miRNA precursor metabolic products and gain further insight into pre-miRNA processing by completely analyzing high-throughput sequencing data. Highly(More)
The aim of the present study was to obtain comprehensive microRNA (miRNA) profiles of type I [Ishikawa (ISK)] and type II (HEC-1B) human endometrial adenocarcinoma cell lines, utilizing the latest high-throughput sequencing techniques. RNA was extracted from ISK and HEC-1B cell lines. Sequencing results were obtained from a next-generation sequencing(More)
3′ addition events in miRNAs are widely detected and may contribute to miRNA stability, but little is known about details of the events in miRNA gene clusters and families. Here, we performed a comprehensive analysis of isomiR expression patterns and 3′ additions in miRNA gene clusters and families by analyzing high-throughput sequencing dataset. According(More)
OBJECTIVE Recently, microRNAs (miRNAs) had been shown as potential important regulators in pregnancy. Circulating miRNAs are considered as potentially useful non-invasive biomarkers for the diagnosis of pregnancy-related disease and congenital disorders, but maternal peripheral blood miRNAs expression profile in pregnancy remains less investigated. We thus(More)
BACKGROUND Lung cancer is the leading cause of cancer deaths in China. Non-small cell lung cancer (NSCLC) is the major type of lung cancer. OBJECTIVES The aim of our study was to characterize the expression profiles of miRNAs in serum and tissue of NSCLC at the same time, and to find more accurate relationship of miRNAs between serum and tissue.(More)