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Reverse transcriptase polymerase chain reaction (RT-PCR) for the diagnosis of rabbit hemorrhagic disease virus (RHDV) was developed by examining sensitivity and specificity. Samples from rabbits infected with rabbit hemorrhagic disease (RHD) were examined to investigate the distribution of the virus in the body. The results showed that the RT-PCR method had(More)
In order to validate the immune-enhancement efficacy of compound Chinese herbal medicinal ingredients (cCHMIs), made with astragalus polysaccharide (APS), epimedium polysaccharide (EPS), propolis flavone (PF) and ginsenosides (GS), as immune potentiator or vaccine adjuvants for rabbits, the effects of two cCHMIs on rabbit lymphocyte proliferation and(More)
To explore the capacity and immunogenicity of virus-like particles (VLPs) of rabbit hemorrhagic disease virus (RHDV) accommodating foreign amino acid sequences, integrations were performed at the following four locations of the structural protein VP60 of RHDV using the OVA257-264 CD8+ T cell epitope (SIINFEKL): (1) inserting at the N-terminus of the VP60(More)
To investigate the genetic variability and evolution of rabbit hemorrhagic disease virus (RHDV) strains in China, VP60 gene sequences of eight new isolates collected from farms with RHD occurrences in China between 2009 and 2014 were analyzed, and compared with the reference sequence of the vaccine strain WF/China/2007. We conducted a comprehensive analysis(More)
Rabbit haemorrhagic disease, caused by rabbit hemorrhagic disease virus (RHDV), results in the death of millions of adult rabbits worldwide, with a mortality rate that exceeds 90%. The sole capsid protein, VP60, is divided into shell (S) and protruding (P) domains, and the more exposed P domain likely contains determinants for cell attachment and antigenic(More)
During infection host histo-blood group antigens (HBGAs) act as attachment factors that interact with rabbit hemorrhagic disease virus (RHDV) and participate in the infectious process. In the present study, baculovirus expressing recombinant RHDV capsid protein (VP60r) as a vaccine immunogen was used to test its antigenicity and immunogenicity via(More)
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