Histone demethylation by a family of JmjC domain-containing proteins
The JmjC domain is identified as a novel demethylase signature motif and a protein demethylation mechanism that is conserved from yeast to human is uncovered.
Role of Histone H3 Lysine 27 Methylation in X Inactivation
It is demonstrated that transient recruitment of the Eed-Ezh2 complex to the inactive X chromosome (Xi) occurs during initiation of X inactivation in both extraembryonic and embryonic cells and is accompanied by H3-K27 methylation.
G9a-mediated irreversible epigenetic inactivation of Oct-3/4 during early embryogenesis
Genetic studies show that epigenetic changes actually have an important role in the inhibition of Oct-3/4 re-expression, thereby preventing reprogramming.
Recognition of Histone H3 Lysine-4 Methylation by the Double Tudor Domain of JMJD2A
This study provides mechanistic insights into recognition of methylated histone tails by tudor domains and reveals the structural intricacy of methyl-lysine recognition by two closely spaced effector domains.
PLU-1 is an H3K4 demethylase involved in transcriptional repression and breast cancer cell proliferation.
Purification and Functional Characterization of SET8, a Nucleosomal Histone H4-Lysine 20-Specific Methyltransferase
Ring1b-mediated H2A Ubiquitination Associates with Inactive X Chromosomes and Is Involved in Initiation of X Inactivation*
- Jia Fang, Taiping Chen, B. Chadwick, E. Li, Yi Zhang
- BiologyJournal of Biological Chemistry
- 17 December 2004
Evidence is provided that supports H2A ubiquitination as a novel epigenetic marker for the inactive X chromosome (Xi) and links H2a ubiquitinations to initiation of X inactivation and the association of Ring1b and ubH2A with Xi is mitotically stable in non-differentiated TS cells.
Methyl‐H3K9‐binding protein MPP8 mediates E‐cadherin gene silencing and promotes tumour cell motility and invasion
A model by which MPP8 recognizes methyl‐H3K9 marks and directs DNA methylation to repress tumour suppressor gene expression and, in turn, has an important function in epithelial‐to‐mesenchymal transition and metastasis is suggested.
MPP8 mediates the interactions between DNA methyltransferase Dnmt3a and H3K9 methyltransferase GLP/G9a.
Findings provide a molecular explanation, at least in part, for the co-occurrence of DNA methylation and H3K9 methylation in chromatin.
The Saccharomyces cerevisiae Histone Demethylase Jhd1 Fine-Tunes the Distribution of H3K36me2
- Jia Fang, Gregory J. Hogan, Gaoyang Liang, J. Lieb, Yi Zhang
- BiologyMolecular and Cellular Biology
- 30 April 2007
These studies establish JHD1 as a histone demethylase in budding yeast and suggest that Jhd1 functions to maintain the fidelity of histone methylation patterns along transcription units.