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PURPOSE Wild-type myocilin is known to be secreted extracellularly, but a significant amount of the protein is also present in the endoplasmic reticulum (ER). The present study was undertaken to address whether intracellular myocilin is a true ER resident protein. METHODS Human wild-type myocilin was adenovirally expressed in human trabecular meshwork(More)
OBJECTIVE Although stem cell factor (SCF) has been shown to play a critical role in hematopoiesis, gametogenesis, and melanogenesis, the function of SCF in the regulation of vascular integrity has not been studied. APPROACH AND RESULTS We demonstrated that SCF binds to and activates the cKit receptor in endothelial cells, thereby increasing the(More)
PURPOSE Stem cell factor (SCF) has recently demonstrated activity as a novel endothelial permeability factor that contributes to the development of diabetes-induced hyperpermeable retinal vasculature. This study investigated the therapeutic potential of masitinib, a pharmacologic inhibitor of the SCF receptor cKit, for prevention of diabetes-induced(More)
To understand the regulatory mechanisms governing glucocorticoid-mediated MYOC induction in human trabecular meshwork (HTM) cells, the expression and degradation of MYOC mRNA were quantified in HTM cells by Northern blot analysis, and the transcriptional activity of constructs containing variable lengths of putative MYOC promoters was assessed by luciferase(More)
We previously reported the successful establishment of embryonic stem cell (ESC)-like multipotent spermatogonial stem cells (mSSCs) from neonatal mouse testis. Here, we examined the ability of mSSCs to differentiate into vascular endothelial cells and smooth muscle cells, and compared to that of mouse ESCs. We used real-time reverse transcriptase polymerase(More)
This study demonstrates the application feasibility of electrochemical impedance spectroscopy (EIS) in measuring estrogen (17β-estradiol) in gas phase. The present biosensor gives a linear response (R(2) = 0.999) for 17β-estradiol vapor concentration from 3.7 ng/L to 3.7 × 10(-4) ng/L with a limit of detection (3.7 × 10(-4) ng/L). The results show that the(More)
PURPOSE Stem cell factor (SCF) has been recently acknowledged as a novel endothelial permeability factor. However, the mechanisms by which SCF-induced activation of the SCF cognate receptor, cKit, enhances endothelial permeability have not been fully elucidated. This study aimed to investigate the role of Src in SCF-induced breakdown of the blood-retinal(More)
T he endothelium lining the blood vasculature forms an interface between the blood and tissues and plays a crucial role in controlling the blood-to-tissue movement of plasma proteins, solute, and water. Under normal physiological conditions, transvascular exchange is modulated by hydrostatic and oncotic forces, the concentration gradient of solutes acting(More)
PURPOSE To assess whether the expression of heat shock protein 72 (Hsp72) protects rat retinal ganglion cells (RGC-5) from apoptotic cell death. METHODS Hsp72 expression in RGC-5 cells transduced with replication-deficient recombinant adenovirus was analyzed by Western blot analysis and immunofluorescence. The effect of Hsp72 expression on(More)
We report the fabrication of a novel titania membrane of the dual-pore system that is strategically designed and prepared by a two-step replication process and sol-gel reaction. The primary nanoporous channel structure is fabricated by the cage-like PMMA template (CPT) obtained from the nanoporous alumina membrane and the secondary mesoporous structure is(More)
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